Method for rapidly picking otolith of juvenile puffer fish

Abstract

The invention provides a method for rapidly picking otolith of juvenile puffer fish. The method firstly separates the heads and bodies of the juvenile puffer fish so as to obtain the heads, then cutsthe whole lower jaws to obtain the upper jaws and the brains, cuts the fish to the back edge of the eyes from the rear parts of the pharynx to the mouth sections of the heads, and prises the incisions. Thus, the sagitta, lapilli and asteriscus of the juvenile puffer fish can be clearly observed under a microscope; and the three pairs of the otolith can be completely picked with the sequence of picking the asteriscus first and then picking the sagitta and the lapilli. The provided method is simple in operation, easy in grasping and short in time consumption, and can accurately and intactly takeout the three pairs of the otolith; the time of picking the otolith of the juvenile puffer fish can be saved, and picking periods can be shorted; and therefore, the method has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological sample preparation, and in particular relates to a method for quickly extracting otoliths from juvenile puffer fish. Background technique [0002] Otoliths are stones deposited in the labyrinth of the inner eardrum during the growth of bony fishes, which play an important role in the balance and hearing of fishes. The ring patterns in otoliths are very stable in the formation process of morphological structure and chemical composition to record the rich bio-physical-chemical environmental information in the life of individual fish, so otoliths play an important role in fish interspecies identification, group division, Ecotype identification, age estimation and other aspects provide a reference. There are 1 pair of Lapilli, Sagittae and Asteriscus in the utricle, sacculus and stellium of the teleost inner ear respectively. [0003] In recent years, the use of otolith microstructural morphologi...

AI Technical Summary

Problems solved by technology

Finally, the back half of the head of puffer fish is like a rectangle, which is different from the side-flat fish. If it opens on the back obliquely above the eyes, it will easily cause the rupture of the otoliths of juvenile puffer fish, and it is difficult to ensure the integrity of the otoliths.

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