Talaromyces sp FL15 and application thereof in mediating nano-silver biosynthesis

A technology of biosynthesis and cyanobacteria, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve environmental pollution and other problems, and achieve the effects of high synthesis efficiency, easy production scale-up, and high yield

Active Publication Date: 2020-12-18
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When preparing nano-silver by chemical method, in order to prevent the agglomeration of nano-silver, polyvinylpyrrolidone, polyvinyl alcohol, chitosan polymer or silver dodecyl sulfate are usually added as protective agents, and whether the prepared nano-silver is safe is still a big question. At the same time, the reagents cause great pollution to the environment

Method used

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  • Talaromyces sp FL15 and application thereof in mediating nano-silver biosynthesis
  • Talaromyces sp FL15 and application thereof in mediating nano-silver biosynthesis
  • Talaromyces sp FL15 and application thereof in mediating nano-silver biosynthesis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Identification of endophytic fungi:

[0072] Observation of strain morphology: inoculate the activated FL15 strains into PDA solid plate medium, YES solid plate medium and CYA solid plate medium and culture them at 28°C for 7 days, observe the colony morphology (such as figure 1 shown); and observe the shape of the mycelium with a microscope (such as figure 2 shown).

[0073] Depend on figure 1 It can be seen that the strain FL15 was cultured on CYA medium at 28°C for 7 days, and the diameter of the colony could reach 35-40mm. flat back ( figure 1 a, 1b). Cultivated on YES medium at 28°C for 7 days, the diameter of the colony can reach 36-40 mm, white velvet, flat surface, slightly raised in the middle, and the center of the colony on the back can be seen as light yellow with white edges ( figure 1 c, 1d). Cultivated on PDA medium at 28°C for 7 days, the diameter of the colony can reach 46-50mm, the colony is light yellow velvety, with a raised center, no droplet...

Embodiment 2

[0092] Biosynthetic nano silver

[0093] (1) Activation of the bacterial strain: Inoculate the FL15 stored on the inclined surface on the PDA solid medium in the ultra-clean bench, place it in a light incubator at 28°C, and cultivate it for 4 days. At this time, the diameter of the colony reaches 60mm;

[0094] (2) Preparation of fermentation broth: Inoculate the activated bacterial strain FL15 into a 500mL Erlenmeyer flask containing 200mL PDB liquid medium in an ultra-clean bench, place it at 28°C, and cultivate it in a shaker at 160rpm for 7 days to obtain a fermentation broth;

[0095] (3) Preparation of the secondary fermentation liquid: the fermented liquid cultivated in step (2) is filtered with a Buchner funnel to obtain the thalline, and the thalline is washed 4 times with deionized water to obtain the wet thalline, and the wet thalline is measured The water content was 10.51%. The obtained wet cells were dispersed in 100 mL of deionized water per 20 g of cells, and p...

Embodiment 3

[0098] Biosynthetic nano silver

[0099] (1) Activation of the strain: Inoculate the FL15 stored on the inclined surface on the PDA solid medium in the ultra-clean bench, place it in a light incubator at 30°C, and cultivate it for 3 days, and the diameter of the colony reaches 60mm;

[0100] (2) Preparation of fermentation broth: Inoculate the activated bacterial strain FL15 into a 500mL Erlenmeyer flask containing 200mL PDB liquid medium in an ultra-clean bench, place it at 28°C, and cultivate it in a shaker at 180rpm for 5 days to obtain a fermentation broth;

[0101] (3) Preparation of the secondary fermentation liquid: the fermentation liquid cultivated in step (2) is filtered with a Buchner funnel to obtain the thalline, and the thalline is washed 5 times with deionized water to obtain the wet thalline, the wet thalline contains The amount of water is 10.51%. Disperse the obtained wet bacteria in 100 mL of deionized water per 20 g of bacteria, and put them in a shaker at ...

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Abstract

The invention belongs to the technical field of biosynthesis, and provides a strain of Talaromyces sp FL15 and application thereof in mediating nano-silver biosynthesis. The Talaromyces sp FL15 provided by the invention is Talaromyces sp, the strain is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan City, China, the preservation date is October 17, 2019, and the preservation number is CCTCC NO: M 2019832. The Talaromyces sp FL15 is found in huperzia serrata endophytic fungi, and the fungi are found to have the ability of mediating nano-silver synthesis. By optimizing synthesis conditions, the obtained nano-silver has high yield, smaller size and obvious bacteriostatic effect.

Description

technical field [0001] The invention relates to the technical field of biosynthesis, in particular to a cyanobacterium FL15 and its application in mediating nano-silver biosynthesis. Background technique [0002] Nano-silver particles refer to simple silver whose size is in the range of 1-100 nm in one-dimensional space. Nano-silver particles have special properties that many macroscopic materials do not have, such as: small size effect, surface effect, quantum size effect and macroscopic quantum tunneling effect. As a new emerging material, nano-silver has broad application prospects in ion detection, chemical detection, and sensing. Because nano-silver has safe antibacterial and bactericidal effects, daily necessities containing nano-silver are more popular with the public. [0003] Traditional nano silver preparation methods are mainly physical and chemical methods. Preparation of nano-silver by physical method has high equipment cost and low yield. When preparing nan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P3/00C12R1/645
CPCC12N1/14C12P3/00C12R2001/645C12N1/145
Inventor 肖依文朱笃张文慧吴文婷汪涯高波良张志斌
Owner JIANGXI SCI & TECH NORMAL UNIV
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