A kind of kumquat essential oil and its preparation method and application

A technology of kumquat essential oil and tangerine essential oil, which is applied in the field of traditional Chinese medicine, can solve the problems of target organ damage and side effects, which cannot be ignored, and achieve the effect of reducing vasoconstrictive pressure, reducing target organ damage, and definite curative effect

Active Publication Date: 2021-11-23
JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the treatment of hypertension is a long-term process, and the side effects of target organ damage cannot be ignored.

Method used

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  • A kind of kumquat essential oil and its preparation method and application
  • A kind of kumquat essential oil and its preparation method and application
  • A kind of kumquat essential oil and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of Kumquat Essential Oil

[0029] 1. Kumquat raw materials: purchased from the Suichuan planting base in Jiangxi Province, identified and reported as the fruit of Fortunella Crassifolia Swingle, a rutaceae kumquat plant, identified by Mr. Liu Yong from the School of Pharmacy, Jiangxi University of Traditional Chinese Medicine.

[0030] 2. Preparation method 1) Pre-treatment: after cleaning the fresh kumquats, pulverize;

[0031] 2) Dry steam extraction: Weigh 12 kg of kumquat pulverized in step 1 and carry out dry steam extraction in the essential oil extraction system device, the extraction time is 2h, stop the instrument, and after the steam is condensed, extract the layered liquid with a 10ml syringe, Collect the essential oil layer to obtain crude kumquat essential oil;

[0032] 3) Refining: put the crude kumquat essential oil obtained in step 2 in a brown bottle, seal the bottle cap with a parafilm, refrigerate at 4°C in the refrigerator for one night,...

Embodiment 2

[0034] Determination of active components in kumquat essential oil

[0035] 1. Experimental procedure

[0036] Gas chromatography conditions: Agilent 19091s-433:93.92873 column, 30m x 0.25mm x 0.25μm. The inlet temperature was 250 °C, the total flow was 19.6 mL / min, the split ratio was 20:1, and the column flow was 1 mL / min. The interface temperature is 280°C. The heating program was maintained at 40 °C for 2 min, raised to 250 °C at 10 °C / min, held for 2 min, and then raised to 280 °C at 20 °C / min, held for 1 min. The carrier gas is high-purity helium. The injection volume was 1 μL.

[0037] Mass spectrometry conditions: EI ionization source, electron energy of 70 eV, ion source temperature of 230 °C, and quadrupole temperature of 150 °C. The measurement method is full scan, the mass range is 35-550u, the multiplier voltage is 1482V, and the filament emission current is 34.6μA. The solvent delay time was 2.0 min.

[0038] Qualitative and quantitative: The NIST11.L spec...

Embodiment 3

[0042] Animal test for prevention and treatment of lowering blood pressure

[0043] The systolic blood pressure, serum angiotensin Ⅱ, aldosterone and vascular endothelial relaxation factor of rats were investigated with a single dose of kumquat essential oil 0.5ml / kg.

[0044] 1. Experimental protocol

[0045] 1.1 Drug preparation

[0046] Take the kumquat essential oil prepared in Example 1 and dilute it with an aqueous solution of 2% Tween 80.

[0047] 1.2 Animals

[0048] SPF grade SD rats, male, body weight (180±10) g, 30 were purchased from the Experimental Animal Center of Jiangxi University of Traditional Chinese Medicine [SCXK (Gan) 2018-0003].

[0049] Raised in the experimental animal barrier system [SYXK (Gan) 2017-0004], the laboratory temperature is 23±2℃, the relative humidity is 53±3%, the noise is below 60 decibels, the sunshine is 12h, the animals drink water and food freely, and the bedding is changed daily. , when the animal body weight was 180 ± 10 g, a...

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Abstract

The invention discloses a kumquat essential oil, a preparation method and application thereof. Kumquat essential oil is a kind of pure natural substance extracted from kumquat fruit. The present invention obtains kumquat essential oil through dry steam extraction, uses GC-MS to measure the main active components and content in the kumquat essential oil, and then establishes a hypertensive animal model test to prove that the kumquat essential oil and its active components can significantly reduce blood vessel Systolic blood pressure, regulating angiotensin II and other vasodilatory factors, while reducing target organ damage caused by high blood pressure; through cell experiments, it has been proved that it can regulate angiotensin II to induce HUVEC cell viability. Therefore, kumquat essential oil has a good preventive effect on renal hypertension.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to a kumquat essential oil and a preparation method and application thereof. Background technique [0002] With the continuous improvement of people's living standards and major changes in dietary structure, the incidence and death toll of cardiovascular and cerebrovascular diseases in my country are also rising. The antihypertensive rate and control rate were 37.2%, 22.9% and 5.7% respectively. The rate of reaching the blood pressure reduction standard is still lower than that of western developed countries. In addition to raising the awareness of patients on the treatment of hypertension, the research on hypertension drugs is also an urgent need to pay attention. The problem. [0003] Kumquat, also known as Luofu (Oujiang Yizhi), longevity kumquat, milk orange (flower mirror), Gongsun orange, kumquat, etc., is the fruit of the Rutaceae kumquat kumquat Fortune...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/75A61P13/12A61P9/12
CPCA61K36/75A61K2236/13A61K2236/331A61P9/12A61P13/12
Inventor 曾治君刘红宁张吉日木吐吉燕华严小军
Owner JIANGXI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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