Use of Poplar Bud Extract in Preparation of Lipoxygenase Inhibitor

A lipoxygenase and extract technology, which is applied in the field of poplar bud extract as a lipoxygenase inhibitor, can solve the problem that the medicinal and edible value of poplar bud extract is not fully utilized, etc., and achieves improvement of flavor and taste and alleviation of allergies. Symptoms, effect of preventing deterioration

Active Publication Date: 2022-05-03
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Poplar buds are not fully developed branches or inflorescences of Populus plants. The bud fat secreted by them is the main glue source of poplar propolis in my country. It is rich in caffeic acid, p-coumaric acid, quercetin, kaempferol, and allium. Li Su and other flavonoids, phenolic acid active compounds, but the medicinal and edible value of poplar bud extract has not been fully utilized

Method used

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  • Use of Poplar Bud Extract in Preparation of Lipoxygenase Inhibitor
  • Use of Poplar Bud Extract in Preparation of Lipoxygenase Inhibitor
  • Use of Poplar Bud Extract in Preparation of Lipoxygenase Inhibitor

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Embodiment 1

[0020] The preparation method of embodiment 1 poplar bud ethanol crude extract and different fraction elution products

[0021] After pulverizing the poplar buds with a pulverizer, take 10 g of pulverized poplar buds in a beaker, add an appropriate amount of petroleum ether for ultrasonic degreasing, repeat 3 times, and then place the degreased poplar buds in a fume hood overnight to volatilize the residual petroleum ether. Add 100mL of 75% ethanol according to the weight-to-volume ratio of material to liquid of 1:10, ultrasonically assist extraction 3 times, each time for 60min, and suction filter to obtain a clarified ethanol crude extract, which is concentrated by rotary evaporation and freeze-dried to obtain a crude extract of poplar buds things. Weigh a certain amount of poplar bud crude extract, dissolve it in ethanol to prepare a crude extract with a concentration of 10 mg / mL, mix it with AB-8 macroporous resin and stir it magnetically for 2 hours to make it fully adsor...

Embodiment 2

[0023] In order to verify the inhibitory effect of poplar bud extract on lipoxygenase, and to screen the highly active fragments in poplar buds that inhibit lipoxygenase, linoleic acid was used as the reaction substrate, and Fe 2+ Determination of half-inhibitory concentration of poplar bud extract on lipoxygenase by oxidation-xylenol orange chromogenic method. In order to further study the inhibitory mechanism of poplar bud extract on lipoxygenase, the enzyme-catalyzed reaction kinetics was used to judge the reversibility and inhibition type of lipoxygenase inhibition, as follows.

[0024] (1) The poplar bud extract that the present invention extracts and different elution components are carried out lipoxygenase half-inhibitory concentration measurement, compares and analyzes predecessors utilizing XAD-2 macroporous resin separation and purification to obtain the isolate to lipoxygenase at the same time Synthase inhibitory activity. Determination result is as shown in table ...

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Abstract

The invention discloses the use of the poplar bud extract in preparing a lipoxygenase inhibitor, and also discloses a preparation method of the poplar bud extract. The present invention provides for the first time the use of poplar bud extracts in the preparation of lipoxygenase inhibitors. With this use, the poplar bud extracts can be made into food additives, used to improve the flavor and taste of processed products such as fruits and vegetables, and prevent oily foods from becoming inferior. change, prolong its storage period, and may also be used to prepare therapeutic drugs such as asthma and allergic rhinitis, and to relieve allergic symptoms, etc., and has a very wide application prospect.

Description

technical field [0001] The invention relates to the application of plant extracts, in particular to the new application of poplar bud extracts as lipoxygenase inhibitors. Background technique [0002] Lipoxygenase (LOX), also known as lipoxygenase, is a group of redox non-heme ferritin, which can specifically catalyze non-heme ferritin containing cis, cis-1,4-pentadiene structure. Saturated fatty acids (or esters) undergo oxygenation reactions to generate hydroperoxides with conjugated double bonds. LOX exists in various plants, such as cereal seeds, soybeans, peas, and potatoes, with the highest content in soybeans. The presence of lipoxygenase in the food system can cause a decrease in the nutritional quality of food and increase the difficulty of storage, which can lead to bad flavors in processed fruit and vegetable products, and deterioration of oil and oily foods during storage and processing. [0003] The study also found that the arachidonic acid-leukotriene metabo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/76A61P11/06A61P11/02A61P37/08A23L33/105A23L3/3472A61K135/00A61K133/00
CPCA61K36/76A61P11/06A61P11/02A61P37/08A23L33/105A23L3/3472A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23V2002/00A23V2200/16A23V2250/21
Inventor 刘睿熊淑雯唐翠娥赵莉鲁群
Owner HUAZHONG AGRI UNIV
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