Xyz microscope with a vertically translatable carriage

Abstract

The invention discloses an XYZ microscope with a vertically translatable carriage. The invention also relates to an autofocus device for microscopy of a plurality of spatially distributed samples andto a method for autofocus and microscopy of a plurality of spatially distributed biological samples by means of a microscope according to one or more of the preceding claims. The device comprises a microscope and an objective table capable of moving relative to the microscope along the z direction, the device is configured to carry out automatic focusing by evaluating the definition of digital microscopic images of an objective table at a plurality of vertical positions under the assistance of a computer. The microscope is characterized in that the objective table comprises a sliding base anda sample table supported on the sliding base, the sample table can horizontally move relative to the sliding base in the z direction, and the sliding base can horizontally move relative to the microscope in the x direction and the y direction.

Description

technical field [0001] One aspect of the invention relates to an autofocus device for microscopic examination of a plurality of spatially distributed (biological) samples, wherein a stage capable of vertical displacement relative to the microscope includes a A translational sliding seat and a sample stage that can be supported on the sliding seat in the z direction. [0002] A second aspect of the invention relates to a method of autofocusing and microscopic examination of a plurality of spatially distributed biological samples by means of a microscope comprising a stage vertically displaceable relative to the microscope. Background technique [0003] Microscopic examination of biological samples is a common state-of-the-art analytical method that provides extensive insight into the sample. A wide variety of methods can be utilized in this process, such as using transflected light microscopy as well as various forms of optical microscopy, such as fluorescence microscopy. M...

AI Technical Summary

Problems solved by technology

[0010] Purely mechanical drives are usually robust, inexpensive and have greater travel, but often do not provide sufficient precision and repeatability when adjusting position

Also, macromechanical actuators often cannot be fabricated fine enough to be decoupled freely

[0011] In prior art mechanical drives, the precision and repeatability of the mechanical drives are often compromised during operation

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