Tracheal intubation method for mouse

Abstract

The invention discloses a tracheal intubation method for a mouse, and belongs to the field of tracheal intubation. The method comprises the steps: the neck of the mouse leans backwards for 45 degreeswith the head end located on an operation table; two sides of the operation table are each provided with a groove wound with a rubber band; the rubber bands are used for pulling upper incisors of themouse; the operation table is connected with a middle vertical plate; supporting frames are installed at two ends of the upper portion of the middle vertical plate; rubber ropes are wound around two sides of each supporting frame; the rubber ropes pull lower incisors of the mouse to open upper and lower jaw joints of the mouse; and cold light source is used for irradiating a position 0.5-1cm awayfrom the neck of the mouse; the tongue of the mouse is pulled out towards the left side; and bent tweezers a and bent tweezers b prop up the pharyngeal wall of the mouse to complete exposure of the glottis of the mouse. The invention provides the trachea intubation method that a special laryngoscope for rodents including mice and the like is not needed, the glottis is exposed more sufficiently, the intubation operation space is larger, and the success rate is higher.

Description

technical field

[0001] The invention relates to the field of tracheal intubation, in particular to a mouse tracheal intubation method. Background technique

[0002] Rodents such as mice and rats, especially mice, can be divided into three types of tracheal intubation methods: invasive, minimally invasive and noninvasive. The invasive tracheal intubation method mainly adopts tracheotomy, in which a tracheal tube is inserted, and a ventilator is connected externally, which is mainly suitable for acute animal experiments. Minimally invasive tracheal intubation mainly uses neck skin incision to expose the trachea, and intubation is carried out through the mouth under the condition of looking directly at the trachea. This method can be used for chronic animal experiments, such as the preparation of a mouse model of myocardial infarction. The non-invasive tracheal intubation method requires the use of a special laryngoscope. The endotracheal tube is sent into the mouse trachea un...

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