Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for Determination of AMG510 Concentration in Plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

A technique of ultra-high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of ultra-high performance liquid chromatography tandem mass spectrometry to determine the concentration of plasma molecular targeting drug AMG510, can solve the problem that the quantitative detection method has not been reported, and achieve the effect of good ionization effect.

Active Publication Date: 2022-04-26
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the quantitative detection method of AMG 510 by ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS / MS)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for Determination of AMG510 Concentration in Plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
  • Method for Determination of AMG510 Concentration in Plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
  • Method for Determination of AMG510 Concentration in Plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The present invention will be further described in detail below in conjunction with examples, so that those skilled in the art can implement it with reference to the description.

[0032] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

[0033] It should be noted that the experimental methods described in the following embodiments, unless otherwise specified, are conventional methods, and the reagents and materials, unless otherwise specified, can be obtained from commercial sources.

[0034]

[0035] 1 solution preparation

[0036] The volumes and weights used to prepare solutions can be adjusted proportionally, and all solutions are stored at room temperature unless otherwise stated.

[0037] 1.1 Preparation of mobile phase solution

[0038] Organic phase (A): Acetonitrile containing formic acid with a mass fraction of 0.1%...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for measuring the concentration of the molecular targeting drug AMG 510 in plasma by ultra-high performance liquid chromatography tandem mass spectrometry, comprising: preparing a standard curve working solution, preparing an internal standard working solution; Add blank plasma to make up to 20 μL, vortex to make a standard curve plasma sample, add internal standard working solution and methanol, vortex, centrifuge, take supernatant, perform UPLC-MS / MS quantitative analysis, and draw a standard curve; precision pipetting For 20 μL of plasma to be tested, the sample pretreatment method is the same as above, and the concentration of AMG 510 is determined according to the standard curve of the current batch. The present invention measures the blood drug concentration of anti-non-small cell lung cancer molecular targeting drug AMG 510 for the first time, which has high sensitivity, strong specificity, rapidity and good reproducibility, and can be further extended to clinical high-throughput detection or monitoring of AMG 510 concentration, improving Benefit-risk ratio in cancer patients.

Description

technical field [0001] The present invention relates to methods for detecting drug concentrations. More specifically, the present invention relates to a method for measuring the concentration of the molecular targeting drug AMG 510 in plasma by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] In recent years, with the widespread clinical application of anti-tumor molecular targeted drugs, epidermal growth factor receptor (EGFR), anaplastic lymphoma kinase (ALK) rearrangement, vascular endothelial growth factor receptor (VEGFR), etc. Tyrosine kinase inhibitors (TKIs) have significantly improved the benefit-risk ratio in patients with advanced cancer. The KRAS gene, located on chromosome 12, is a proto-oncogene of the RAS family and an important "switch" in the intracellular signal transduction pathway. Once it is turned on, it activates multiple division and proliferation factors, including c-Raf and PI3K. Under normal ci...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045G01N2030/062
Inventor 刘丽宏杜萍安卓玲王国永
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com