The invention relates to an establishment method for hydrocarbon source rock hydrocarbon production rate charts in petroleum resource assessment. The establishment method includes the steps of 1), collecting experimental samples and determining thermal simulation experiment data through experiments; 2), collecting materials; 3), calibrating kerogen generated oil, generated gas and oil-cracking gas kinetic parameters; 4), establishing sedimentary burial history and thermal-history models of hydrocarbon source rocks on a target stratum of a research area; 5), establishing constraint condition of hydrocarbon source rock hydrocarbon production rate; 6), establishing kerogen generated oil, kerogen generated gas, oil generated gas, clean and total gas conversion profile charts on the target stratum of the research area; 7), checking whether dynamics geological extrapolation results meet requirements or not; 8), putting forwards part of hydrocarbon expulsion mode adjustment coefficients, evaluating hydrocarbon production rate curves of the partial hydrocarbon expulsion model, and establishing the hydrocarbon source rock hydrocarbon production rate charts including three modes, namely the mode of complete hydrocarbon expulsion, the mode of partial hydrocarbon expulsion and the mode of no hydrocarbon expulsion. The shortcoming in an original calculating method and experiment simulated data of the hydrocarbon source rock hydrocarbon production rate is overcome, and the hydrocarbon source rock hydrocarbon production rate at the target stratum can be accurately and reasonably determined according to oil field exploration practice by oil field workers.