Method for determining AMG 510 concentration in blood plasma by ultra-high performance liquid chromatography-tandem mass spectrometry

A technology of ultra-high performance liquid chromatography and tandem mass spectrometry, which is applied in the field of ultra-high performance liquid chromatography and tandem mass spectrometry to determine the concentration of the plasma molecular targeting drug AMG510, which can solve the problems of quantitative detection methods that have not been reported, and increase the risk and burden of invasive blood collection , reduce benefit, increase sensitivity effect

Active Publication Date: 2021-01-01
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

However, there is no report on the quantitative detection method of AMG 510 b...

Method used

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  • Method for determining AMG 510 concentration in blood plasma by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining AMG 510 concentration in blood plasma by ultra-high performance liquid chromatography-tandem mass spectrometry
  • Method for determining AMG 510 concentration in blood plasma by ultra-high performance liquid chromatography-tandem mass spectrometry

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example 1

[0035] 1 solution preparation

[0036] The volumes and weights used to prepare solutions can be adjusted proportionally, and all solutions are stored at room temperature unless otherwise stated.

[0037] 1.1 Preparation of mobile phase solution

[0038] Organic phase (A): Acetonitrile containing formic acid with a mass fraction of 0.1%.

[0039] Water phase (B): water containing formic acid with a mass fraction of 0.1%.

[0040] Mobile phase selection: In order to meet the requirements of low quantitative detection range in the experiment, we investigated the organic phase (such as methanol, acetonitrile, etc.) and aqueous phase in various commonly used mobile phases, and tried to add different proportions of solution enhancers (such as 0.01 % formic acid, 0.05% formic acid, 0.1% formic acid, 0.01% acetic acid, 0.1% trifluoroacetic acid, 5mM ammonium formate, 2mM ammonium acetate, etc.). It was found that when formic acid was added to the organic phase and the aqueous phase...

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Abstract

The invention discloses a method for determining the concentration of a molecular targeting drug AMG 510 in blood plasma through ultra-high performance liquid chromatography-tandem mass spectrometry.The method comprises the following steps: preparing a standard curve working solution and an internal standard substance working solution; adding 0-5 microliters of standard curve working solution into blank plasma for making up to 20 microliters, performing vortex to prepare a standard curve plasma sample, adding the internal standard substance working solution and methanol, performing vortex andcentrifugation, taking supernatant, performing UPLC-MS/MS quantitative analysis, and drawing a standard curve; precisely sucking 20 microliters of to-be-detected plasma, and determining the concentration of AMG 510 according to the standard curve of the current batch by the same sample pretreatment method. The blood concentration of the non-small cell lung cancer resistant molecular targeted drugAMG 510 is determined for the first time, the sensitivity is high, the specificity is high, the speed is high, the reproducibility is good, the method can be further popularized to clinical high-throughput detection or monitoring of the AMG 510 concentration, and the benefit risk ratio of cancer patients is increased.

Description

technical field [0001] The present invention relates to methods for detecting drug concentrations. More specifically, the present invention relates to a method for measuring the concentration of the molecular targeting drug AMG 510 in plasma by ultra-high performance liquid chromatography tandem mass spectrometry. Background technique [0002] In recent years, with the widespread clinical application of anti-tumor molecular targeted drugs, epidermal growth factor receptor (EGFR), anaplastic lymphoma kinase (ALK) rearrangement, vascular endothelial growth factor receptor (VEGFR), etc. Tyrosine kinase inhibitors (TKIs) have significantly improved the benefit-risk ratio in patients with advanced cancer. The KRAS gene, located on chromosome 12, is a proto-oncogene of the RAS family and an important "switch" in the intracellular signal transduction pathway. Once it is turned on, it activates multiple division and proliferation factors, including c-Raf and PI3K. Under normal ci...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045G01N2030/062
Inventor 刘丽宏杜萍安卓玲王国永
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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