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Picoliter-scale single droplet generation method and device

A production method and a production device technology, which are applied to chemical instruments and methods, liquid separation into beads and granulation, measuring tubes/pipettes, etc., can solve the problems of large difference in results, difficult control, high cost, etc., and achieve liquid The effect of high droplet production efficiency, improved control accuracy and improved production efficiency

Inactive Publication Date: 2021-01-08
NINGBO UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The structure is complex, the cost is high, the droplet volume is large, and the micro-droplets cannot be stably produced
When single-cell extraction is performed, the single-cell encapsulation rate is low; small closed structures are difficult to open; small-volume droplets have low precision control capabilities and complex equipment; human interference is large, control is difficult, and stability is poor. When the analysis is carried out separately, the results are highly variable, the repeatability is low, and the operation is complicated, etc.

Method used

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  • Picoliter-scale single droplet generation method and device
  • Picoliter-scale single droplet generation method and device
  • Picoliter-scale single droplet generation method and device

Examples

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Effect test

Embodiment 1

[0035] figure 1 A flow chart of the method for producing a picoliter single droplet in Embodiment 1 of the present invention is schematically shown, as figure 1 As shown, the method for producing a picoliter single droplet comprises the following steps:

[0036] (A1) By studying the correlation between the volume of a single droplet and the inner diameter of the bottom of the capillary, the lifting speed of the capillary, and the gas pressure pushing the liquid in the capillary, such as Figure 2-4 As shown, thereby establishing the mapping relationship between the volume of a single droplet and the capillary parameters, the gas pressure pushing the liquid in the capillary, and the capillary lifting velocity;

[0037] (A2) Using the mapping relationship to obtain the target value of the control parameter corresponding to the target volume droplet, the control parameter includes the gas pressure and the lifting speed;

[0038] (A3) adjusting the gas pressure to a target valu...

Embodiment 2

[0056] An application example of the method and device for generating a picoliter single droplet according to Embodiment 1 of the present invention in single cell detection.

[0057] In this application example, the capillary is made of borosilicate glass, one end of which is open to the gas source, and the inner diameter of the bottom of the capillary is 20 μm;

[0058] The pressure control unit is arranged between the capillary and the gas source, and is used to adjust the pressure of the gas entering the capillary;

[0059] The method for producing a picoliter single droplet according to an embodiment of the present invention comprises the following steps:

[0060] (A1) Establish a mapping relationship between the volume of a single droplet and capillary parameters, the gas pressure that promotes the liquid in the capillary, and the capillary lift velocity:

[0061] V is the drip volume, R is the inner diameter of the capillary bottom, ω is the taper of the capillary bot...

Embodiment 3

[0069] An application example of the method and device for generating a picoliter single droplet according to Embodiment 1 of the present invention in single cell detection.

[0070] In this application example, the capillary is made of borosilicate glass, one end of which is open to the gas source, and the inner diameter of the bottom of the capillary is 15 μm;

[0071] The pressure control unit is arranged between the capillary and the gas source, and is used to adjust the pressure of the gas entering the capillary;

[0072] The method for producing a picoliter single droplet according to an embodiment of the present invention comprises the following steps:

[0073] (A1) Establish a mapping relationship between the volume of a single droplet and capillary parameters, the gas pressure that promotes the liquid in the capillary, and the capillary lift velocity:

[0074] V is the drip volume, R is the inner diameter of the capillary bottom, ω is the taper of the capillary bot...

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Abstract

The invention provides a picoliter-scale single droplet generation method and device. The picoliter-scale single droplet generation method comprises the following steps of: (A1) establishing a mappingrelationship between single droplet volume and capillary parameters, the gas pressure for pushing the liquid in a capillary and the capillary lifting speed; (A2) acquiring control parameters corresponding to the target volume liquid drops by utilizing the mapping relationship, wherein the control parameters comprise the gas pressure and the lifting speed; (A3) adjusting the gas pressure to a target value of the gas pressure; (A4) enabling the liquid at the bottom end of the capillary to be in contact with a bottom surface medium, and enabling the liquid in the capillary to flow downwards; and(A5) moving the bottom end of the capillary upwards to be separated from the bottom surface medium, and forming picoliter-scale single droplets on the bottom surface medium. The method has the advantages of high precision, high efficiency and the like.

Description

technical field [0001] The invention relates to liquid control, in particular to a method and device for generating picoliter single liquid droplets. Background technique [0002] Cells are the basic units that make up living organisms. To understand the changes and behaviors of individual cells at various stages in a complex and changeable environment, methods for single-cell analysis are required. Mass spectrometry is a method for simultaneous analysis of multiple components. According to the different molecular weights of various components in cells, spectral peaks arranged according to mass numbers can be formed in mass spectrometry, and further through multi-stage mass spectrometry analysis, various components in cells can be obtained. molecular information. Since mass spectrometry does not require labels and does not need to know the information of the molecules to be tested in advance, it can quickly identify various unknown components in cells and obtain the omics i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J2/02B01J13/02B01L3/02
CPCB01J2/02B01J13/02B01L3/0241
Inventor 闻路红闫明月陈安琪甘剑勤毕磊李文
Owner NINGBO UNIV
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