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Droplet generation method and device

A method for producing liquid droplets, which is applied to chemical instruments and methods, measuring tubes/pipettes, and laboratory containers. It can solve problems such as inability to release, limited detection methods, and small specific surface area of ​​liquid droplets. Improve control accuracy, increase production efficiency, and achieve high control accuracy

Active Publication Date: 2022-04-05
CHINA INNOVATION INSTR CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The design of the channel of the microfluidic chip restricts the release of the extraction droplets in the chambers on both sides of the channel, so that the detection method is limited to the optical method, and it is difficult to give further detailed information on the target substance
Or the droplet is an embolism type, and the specific surface area of ​​the droplet is small, which is not conducive to liquid-liquid extraction
The spotting of a single droplet using a capillary needle is estimated based on the diameter of the capillary needle and the surface tension of the solution, and it is impossible to accurately obtain a skin-upgraded single droplet.

Method used

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  • Droplet generation method and device

Examples

Experimental program
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Effect test

Embodiment 1

[0033] figure 1 Schematically provides a flow chart of the droplet generation method in Embodiment 1 of the present invention, as figure 1 Shown, the generation method of described droplet comprises the following steps:

[0034] (A1) Obtaining initial parameters of the capillary, the initial parameters including the inclination angle of the capillary relative to the substrate;

[0035] figure 2 Schematically shows the cross-sectional structure of the capillary, such as figure 2 As shown, the capillary is a hollow structure with openings at both ends, the liquid level in the capillary is horizontal, and the liquid in the suspended capillary will not drip when it is not subjected to external force; is the angle between the central axis of the capillary and the generatrix of the inner surface, and θ is the maximum value of the angle between the generatrix and the liquid surface inside the capillary;

[0036] (A2) Utilize the mapping relationship to obtain the target parame...

Embodiment 2

[0073] An application example of the droplet generation method and device according to Embodiment 1 of the present invention in single cell detection.

[0074] In this application example, the inner and outer walls of the capillary are treated as follows: soak the capillary in 8% to 12% NaOH solution and Piranha solution for 2 to 10 minutes each, and then soak the capillary in 18% to 22% NaOH solution. Put in the ethanol solution of chlorodimethylsilane for 1 to 2 hours, wash with clean water, and then place the capillary in an oven at 100 to 150 degrees and bake for 30 to 60 minutes to remove stress.

[0075] The surface treatment of the substrate is as follows:

[0076] After mixing the silica sol and the nano-silica solution modified by sulfonic acid groups in a certain ratio (preferably 1:16), coat it on the surface of the substrate, and bake it in an oven at 100-150 degrees for 30-60 minutes; the substrate The glass coefficient k of the surface 1 = 0.89.

[0077] Use t...

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Abstract

The present invention provides a method and device for generating a droplet, the method for generating a droplet includes the following steps: (A1) obtaining initial parameters of the capillary, the initial parameters including the inclination angle of the capillary relative to the substrate; (A2 ) using a mapping relationship to obtain a target parameter corresponding to the radius of the target droplet, the mapping relationship being the correspondence between the radius of the droplet and a control parameter; the control parameter includes the inclination angle of the capillary relative to the substrate and the flow rate of the liquid in the capillary; (A3) adjusting the initial parameters of the capillary to the target parameters; (A4) the liquid at the bottom of the capillary contacts the substrate, and the liquid in the capillary flows downward; (A5) the capillary The bottom end moves up to disengage from the substrate on which droplets form. The invention has the advantages of high precision and high efficiency.

Description

technical field [0001] The invention relates to liquid control, in particular to a method and device for generating liquid droplets. Background technique [0002] Cells are the basic units that make up living organisms. To understand the changes and behaviors of individual cells at various stages in a complex and changeable environment, methods for single-cell analysis are required. Mass spectrometry is a method for simultaneous analysis of multiple components. According to the different molecular weights of various components in cells, spectral peaks arranged according to mass numbers can be formed in mass spectrometry, and further through multi-stage mass spectrometry analysis, various components in cells can be obtained. molecular information. Since mass spectrometry does not require labels and does not need to know the information of the molecules to be tested in advance, it can quickly identify various unknown components in cells and obtain the omics information of pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/02
CPCB01L3/0241
Inventor 刘云闻路红毕磊甘剑勤洪欢欢李文
Owner CHINA INNOVATION INSTR CO LTD
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