Hepatitis C virus detection kit

Abstract

The invention discloses a hepatitis C virus detection kit. The detection kit comprises two pairs of amplification primers and a multiplex PCR mixed reaction liquid, wherein the amplification primers are used for amplifying obese gene polymorphic sites and comprise an rs8099917 site and an rs12979860 site, and each pair of primers corresponds to an upstream and downstream regions of one SNP site respectively. According to the hepatitis C virus detection kit, a multiplex PCR amplification method is adopted, two important SNP sites of IL-28B are amplified at the same time, amplified site combinations can be flexibly selected according to actual needs to achieve the effect of one kit with multiple functions, primers in the kit are high in specificity and low in mismatch rate, and the primer isreasonable in length and the Tm value design. The amplification product is convenient to detect and isolate. Multiple target products are amplified once, so that the amplification efficiency can be greatly improved, and the amplification cost can be reduced. The detection kit can be extended to other SNP site detection, and has a good popularization value.

Description

technical field [0001] The invention relates to a detection kit for hepatitis C virus, which belongs to the field of detection kits. Background technique [0002] Hepatitis C virus (HCV) is an important type of chronic hepatitis and one of the most widespread infectious diseases in the world. Neglected by patients is one of the main causes of liver cirrhosis, liver cancer and liver failure. The international standard regimen for the treatment of CHC is pegylated interferon α (Peg-IFNα) combined with ribavirin (RBV). In recent years, studies have shown that the clinical efficacy of CHC antiviral therapy is not only affected by viral load, age, In addition to the influence of race and liver morphology, it is also highly correlated with the single nucleotide polymorphism of interleukin-28B (IL-28B) located on human chromosome 19 encoding interferon λ3, but most reports focus on IL-28B rs8099917 site, and the predictive value of IL-28B on the curative effect and prognosis of h...

AI Technical Summary

Problems solved by technology

The international standard regimen for the treatment of CHC is pegylated interferon α (Peg-IFNα) combined with ribavirin (RBV). In recent years, studies have shown that the clinical efficacy of CHC antiviral therapy is not only affected by viral load, age, In addition to the influence of race and liver morphology, it is also highly correlated with the single nucleotide polymorphism of interleukin-28B (IL-28B) located on human chromosome 19 encoding interferon λ3, but most reports focus on IL-28B rs8099917 site, and the predictive value of IL-28B on the curative effect and prognosis of hepatitis C treatment is still in the preliminary research stage

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