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A lipid droplet induction and imaging reagent and its preparation method and application

A technology of reagents and lipid droplets, which is applied in the field of lipid droplet induction and imaging reagents and its preparation, can solve the problems of poor safety, poor water solubility, and inability to specifically trace lipid droplets, and achieve good cytocompatibility, Low toxicity and good water dispersibility

Active Publication Date: 2022-03-08
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Purpose of the invention: In view of the problems existing in the prior art, the present invention provides a highly efficient lipid droplet induction and fluorescent labeling reagent, which can effectively solve the problem of poor water solubility of existing lipid droplet induction reagents and the inability to specifically trace lipid droplets. and disadvantages such as poor security

Method used

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  • A lipid droplet induction and imaging reagent and its preparation method and application
  • A lipid droplet induction and imaging reagent and its preparation method and application
  • A lipid droplet induction and imaging reagent and its preparation method and application

Examples

Experimental program
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Embodiment 1

[0034] (1) Weigh 10mg of diblasonic acid phosphatide (DOPS, structural formula) figure 2 ) And dissolved in 0.5 ml of chloroform, and the nitrogen was blown up and dried in a vacuum drying box for 4 hours. After adding 1 ml of phosphate buffer oscillated water, a liposome was prepared using a probe ultrasound (163W, 4 minutes), named DOPS liposome;

[0035] (2) Fluorescent phospholipids having an NBD mark with a 0.2 ml concentration of 1 mg / ml and the head is a phosphatidyl racemin group (named NBD-PS, and the structural formula is attached. figure 2 (Solvent is chloroform), and mixed with DOPS (10 mg) dissolved in 0.5 ml of chloroform, and the nitrogen was blown out of the vacuum drying tank in a vacuum drying tank for 4 hours. After adding 1 ml of phosphate buffer hydrated dispersion, a liposome was prepared using a probe ultrasound (163W, 4 minutes), named DOPS / NBD-PS liposome (the structure was attached image 3 .

Embodiment 2

[0037] Different saturation of lipids induced and imaging liposomes:

[0038] The preparation method is the same as that of Example 1, and the DOPS used in Example 1 (2) is replaced with an equal mass of Palmyl phosphatide (POPS).

Embodiment 3

[0040] Lip drop induction and imaging liposomes of different head groups:

[0041] The preparation method is the same as that of Example 1, and the DOPS used in (2) in Example 1 is replaced by equal quality diblasceryl glycerol (DOPG), diblastin phosphatidylinol (DOPI), double Oil liponic acid (DOPA) or laminated diblactyl phosphatidylglycerol (DOCA).

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Abstract

The invention discloses a lipid drop induction and imaging reagent and its preparation method and application. The reagent is a liposome mainly composed of negatively charged unsaturated glycerophospholipids and phospholipids whose tails are marked with fluorescence. The liposome of the present invention has good water dispersibility, can effectively induce cells to generate a large number of lipid droplets without affecting cell activity, and the efficiency of lipid droplet generation is greatly improved compared with existing induction strategies. More importantly, the reagent of the present invention can also perform fluorescent labeling on intracellular lipid droplets, thereby realizing imaging and tracking of lipid droplets.

Description

Technical field [0001] The present invention relates to biotechnology, and more particularly to a lipid droplet induction and imaging reagent and a preparation method thereof. Background technique [0002] Lipid Droplets, LDS is the main storage site of intracellular neutral lipids, which can store triglycerides, sterol esters, and xeroxyl ester, and a multi-functional or capable of protein-containing multi-functional orientation. . Recent studies have found that aliphatic drops are essential for many cellular functions, in addition to fat storage, such as lipid metabolism, membrane biosynthesis, cellular signaling and inflammation. In addition, more and more evidence suggests that cancer is related to abnormalities of lipopters. These important biological functions of lipids are urgently needed to develop reagents that can efficiently regulate and monitor cell lipids. However, the current lipid-induced reagent, such as cyclosporin, is now listed as a kind of carcinogen; and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06G01N21/64
CPCC09K11/06G01N21/6456G01N21/6486C09K2211/1048C09K2211/1011C09K2211/1055C09K2211/1007
Inventor 吴富根蒋耀文高歌
Owner SOUTHEAST UNIV