Metagenome sequencing data automatic analysis method

Abstract

The invention relates to the field of biological information, and discloses a metagenome sequencing data automatic analysis method, which comprises the steps of original offline data filtering and quality control, host genome comparison, intestinal flora species annotation and comparison, intestinal flora metabolic potential analysis, result statistics and output and the like. Python script connection is used in all the steps to achieve automatic analysis, tedious manual operation is reduced, and various errors caused by manual operation are reduced. The analysis content of the automatic analysis method covers most of metagenome analysis content, the analysis requirements of researchers can be met, intermediate results generated in all steps can be output during operation of the process, corresponding data can be subjected to statistical analysis and arrangement, a corresponding chart can be output, browsing of a user is facilitated, and meanwhile, the process operation log records allprocess related information, so that screening can be conveniently carried out when process operation errors occur.

Description

technical field [0001] The invention relates to the field of biological information, in particular to an automatic analysis method for metagenomic sequencing data. Background technique [0002] Metagenome, also known as microbial environmental genome, refers to the sum of all biological genetic materials in the environment. The research object is the genome of microbial populations in environmental samples. Common research methods include functional gene screening, sequencing analysis, etc. In recent years, the relationship between the microbiome and human health has become a research hotspot, especially the metagenomic technology based on high-throughput sequencing has promoted the development of this field. To understand the impact of gut microbes on human health, it is necessary to clarify the intestinal The composition, diversity and function of the microenvironment, etc. The Human Microbiome Project initiated by the National Institutes of Health generated a large numbe...

AI Technical Summary

Problems solved by technology

However, the software usage environment, input and output data types, and data structures used in the above process are not the same, so it is necessary to manually organize and link the step-by-step results, which increases labor costs

[0005] At present, there is no automated analysis platform or open source process for metagenomics to solve the problem of manual sorting and linking of intermediate step-by-step results. Therefore, an automated analysis method for metagenomic sequencing data is designed to cover most of the analysis content required by the market. , can automatically complete the analysis of each part and then count, classify and organize the results, which has practical significance and good application prospects

Images