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Construction method of lumbar intervertebral disc protrusion mouse model

A technology of lumbar intervertebral disc herniation and mouse model, which is applied in the field of biomedicine, can solve the problems of few pain behavioral evaluation methods, short duration, and few transgenic strains of rats, so as to enrich transgenic strains and facilitate genetic manipulation methods Effect

Inactive Publication Date: 2021-02-02
王跃 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this model are as follows: 1) The normal and undegenerated intervertebral disc is used to compress the nerve root, which is inconsistent with the clinical reality of lumbar disc herniation, and the pain symptoms caused by it are relatively mild and the duration is relatively short (only about 1 -2 weeks); 2) There are few pain behavioral evaluation methods based on rats; 3) There are few transgenic rat strains available for research, which is not conducive to in-depth research on the biological mechanism of pain

Method used

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  • Construction method of lumbar intervertebral disc protrusion mouse model

Examples

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Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Injury-induced lumbar disc degeneration

[0030] (1) Animals

[0031] Mice (C57BL / 6); body weight: about 25.0±3.0g; sex: male; age: 2-3 months.

[0032] (2) Equipment and materials

[0033] Orthopedic surgical instruments, microsurgical instruments, anesthetics, puncture needles with depth-limiting devices.

[0034] (3) Method

[0035] 1. Anesthesia

[0036] The animals were weighed and anesthetized by intraperitoneal injection of pentobarbital sodium at a dose of 0.1 mg / g.

[0037] 2. Skin preparation

[0038] The supine position was fixed on the operating table, the abdominal skin was removed, the local area was disinfected twice with povidone iodine, a sterile surgical towel was spread, and the surgical site was exposed.

[0039] 3. Lumbar disc puncture surgery

[0040] (1) Make a 1-1.5cm longitudinal incision in the middle of the lower abdomen, incise the subcutaneous skin, retract the small intestine, and expose the gap between the iliopsoas muscl...

Embodiment 2

[0041] Embodiment 2: Construction of mouse lumbar disc herniation model

[0042] (1) Animals

[0043] Mice (C57BL / 6); body weight: about 25.0±3.0g; gender: male; age: 2-3 months.

[0044] (2) Equipment and materials

[0045] Orthopedic surgical instruments, microsurgical instruments, anesthetics.

[0046] (3) Method

[0047] 1. Anesthesia

[0048] The animals were weighed and anesthetized by intraperitoneal injection of pentobarbital sodium at a dose of 0.1 mg / g.

[0049] 2. Skin preparation

[0050] The patient was fixed on the operating table in the prone position, the skin on the back was removed, the local area was disinfected twice with povidone iodine, a sterile surgical towel was spread, and the surgical site was exposed.

[0051] 3. Lumbar disc puncture surgery

[0052] (1) With the L4 / 5 vertebral body as the center, a posterior midline incision is made with a length of about 1-1.5 cm. Bluntly dissect the left paraspinal muscles to expose the lamina, cut off the ...

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Abstract

The invention discloses a construction method of a lumbar intervertebral disc protrusion mouse model. The method comprises the following steps of 1) performing lumbar intervertebral disc puncture through an abdominal approach to induce lumbar intervertebral disc degeneration; 2) feeding animals for 4 weeks to obtain degenerated lumbar intervertebral disc nucleus pulposus tissues; 3) exposing spinal canal dural sacs and nerve roots through a posterior approach, and placing degenerated intervertebral disc tissues in an intervertebral foramen area to form nerve root compression, so as to obtain the lumbar intervertebral disc protrusion mouse model; and 4) performing behavioral detection to evaluate the performance and degree of lower limb radiation pain. The degenerated intervertebral disc tissues are used for pressing the nerve roots, severe and lasting lower limb radiation pain symptoms can be induced, and the chronic disease course of the clinical lumbar intervertebral disc protrusionis well simulated; and the mouse is used as a model animal, so that a plurality of objective and credible pain behavioral measurement methods and rich transgenic strains are beneficial to deep exploration of a neurobiological mechanism of the lower limb radiation pain of the lumbar intervertebral disc protrusion.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for constructing a mouse model of lumbar disc herniation. Background technique [0002] Lumbar disc herniation is one of the main causes of lumbago and lower extremity radiating pain, which is quite common in clinic. Lumbar and leg pain symptoms caused by lumbar disc herniation seriously impair the quality of life and work ability of patients, and affect economic production. At present, the neurobiological mechanism of pain caused by lumbar disc herniation has not been fully elucidated. An effective and credible animal model of lumbar disc herniation is a key scientific research tool to further elucidate the neural mechanism of low back pain and develop new clinical drugs. [0003] Early related studies mostly used larger animals such as pigs, dogs or rabbits to make models of lumbar disc herniation. These large animal models are expensive and relatively convenient to opera...

Claims

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Application Information

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IPC IPC(8): A01K67/027A61K49/00
CPCA01K67/027A01K2207/30A01K2227/105A61K49/0008
Inventor 王跃陆轩黄嘉炜
Owner 王跃
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