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Insect specific polyamine micromolecular toxin, as well as purification method and application thereof

A purification method and technology of small molecules, applied in chemical instruments and methods, pesticides, organic chemistry, etc., to achieve the effect of strong practicability, low loss rate, and reduced sample loss

Active Publication Date: 2017-08-18
HUNAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are no other reports on the isolation, purification and functional research of the insect-specific small molecule toxin from the spider venom

Method used

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  • Insect specific polyamine micromolecular toxin, as well as purification method and application thereof
  • Insect specific polyamine micromolecular toxin, as well as purification method and application thereof
  • Insect specific polyamine micromolecular toxin, as well as purification method and application thereof

Examples

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Embodiment 1

[0039] An insect-specific polyamine small molecule toxin, the relative molecular weight of the insect-specific polyamine small molecule toxin is 622.3971; its molecular formula is C 28 h 50 N 10 o 6 , its structural formula is as follows:

[0040]

[0041] The method for purifying the above-mentioned insect-specific polyamine small molecule toxins specifically comprises the following steps:

[0042] The first step is the ultrafiltration of venom, specifically: the venom collected by electrical stimulation is separated by ultrafiltration tubes, repeated several times, and the filtrates are combined to obtain a non-protein small molecule toxin mixture containing insect-specific polyamine small molecule toxins . Here, the venom is the venom of the Eurasia grandis; the ultrafiltration membrane in the ultrafiltration tube is a semi-permeable membrane that allows molecules below 3KDa to pass through; the separation is specifically centrifuged at a speed of 8000-12000rpm for 8-1...

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Abstract

The invention provides insect specific polyamine micromolecular toxin (denoted as AVTX-623), wherein the relative molecular weight is 622.3971 and the molecular formula is C28H50N10O6. The AVTX-623 has the following properties: (1) the AVTX-623 can specifically act on an insect sodium ion channel, and the action dose of the AVTX-623 on the insect sodium channel is ten times lower than that of ArgTX-636; (2) the toxicity result on the insect living body indicates that the half effective anesthetic dosage of the AVTX-623 on the insect is 20 times lower than that of the ArgTX-636; (3) for vertebrate NMDA receptor activity, the action dosage of the AVTX-623 is slightly lower than that of the ArgTX-636; (4) the selectivity activity of the AVTX-623 on the insects is much higher than that of the ArgTX-636. The invention further discloses a purification method of the AVTX-623. Pure AVTX-623 can be completely purified from venom of araneus ventricosus through three steps of ultra-filtration, ion-exchange column chromatography and reversed phase high-performance liquid chromatography, the operation steps are simple, the loss rate is low, and the practicability is high. The invention further discloses application of the AVTX-623. The AVTX-623 can be applied to preparation of insecticide or / and a neurobiological reagent and medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an insect-specific polyamine-like small molecule toxin extracted from the venom of E. magna, its purification method and its application. Background technique [0002] The large-bellied garden spider (Araneus ventricosus) is a medium-sized spider that feeds on insects, and belongs to the phylum Arthropoda, Araneus, Aranea, Orbidae, and Orb genus in classification. The garden spider is one of the most widely distributed spiders in the world. There are a large number of toxic components in its venom that can anesthetize or kill insects. Explore the structural and functional characteristics of these insect-specific toxin molecules, and screen out precursor molecules that may be used as neurobiological research tool reagents, drugs and insecticides. [0003] At present, there are very few studies on the venom of the genus Arachnoides. Kawai et al. in Japan obtained the venom extract by ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C279/14C07C277/08A01P7/04A61P43/00A61P25/08A61P25/24A61P25/28
CPCC07C277/08C07C279/14
Inventor 段志贵梁宋平刘凯
Owner HUNAN NORMAL UNIVERSITY
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