The invention discloses an intervertebral foramen microscopic puncture positioner, including a main arm, wherein the top of the main arm is sleeved with a first sleeve, and a fixing bolt is arranged at the bottom of the main arm, the outer wall of the first sleeve is provided with a quickening bolt, and one side of the first sleeve is provided with an auxiliary arm, the outer sleeve of the auxiliary arm is provided with a slide pipe, the bottom of the slide pipe is provided with a beam frame, the inside of the beam frame is provided with a sliding groove, the sliding block is arranged inside the sliding groove, the bottom of the slider is provided with a support pipe, the outer sleeve of the support pipe is provided with a second sleeve, a beam frame is arranged, Chute and slider, a support pipe is fixedly connected with the slider, Then the slider is pushed to make the slider move along the sliding groove inside the beam frame, and the instrument on the supporting tube moves along with the slider, so that the hole mirror puncture can be quickly carried out between different vertebrae of the patient, thereby solving the problem that the position of the hole mirror puncture needle is difficult to be adjusted by the traditional device, and the puncture time is prolonged.