A method for detecting the ecotoxicity of heavy metal polluted soil by using luminescent earthworms

Abstract

The invention belongs to the technical field of soil detection, and discloses a method for detecting the ecological toxicity of heavy metal-contaminated soil by using luminescent earthworms. The method for detecting the ecological toxicity of heavy metal-contaminated soil by using luminescent earthworms includes: using a soil heavy metal detection device to detect soil samples , to obtain the types of heavy metals contained in the soil of the area to be detected; obtain Eisenia chinensis from water peanuts, purify Eisenia chinensis to remove impurities in the body; measure the initial fluorescence value of the earthworm body; put the earthworm into the soil sample Cultivate in medium; collect Eisenia chinensis, and detect the fluorescence value after secreting mucus to obtain the actual fluorescence value; calculate the ecological toxicity of heavy metal polluted soil. The present invention utilizes the principle that heavy metals suppress photoproteins in the body of luminescent earthworms, detects the fluorescence value of the mucus produced before and after activities in the soil, and calculates the average light suppression rate, which takes less time and has higher accuracy.

Description

technical field [0001] The invention belongs to the technical field of soil detection, and in particular relates to a method for detecting the ecological toxicity of heavy metal polluted soil by using luminescent earthworms. Background technique [0002] At present: With the development and progress of human science, a large number of heavy metal pollutants enter the soil environment through various channels, resulting in more and more serious problems of heavy metal pollution in soil. The earthworm test is a common method for diagnosing and identifying the ecological effects of soil pollution. Earthworms are complete soil annelids that accumulate heavy metals through the food chain in the soil. The content of heavy metals in earthworms increases with the deepening of heavy metal pollution. They are the most commonly used indicators for monitoring soil heavy metal pollution and evaluating soil quality. Among earthworms, there is a special kind of earthworms that can emit li...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there are many studies on earthworm ecotoxicity tests, but most of them focus on the impact of pesticides or heavy metal pollution on the mortality of earthworms or the evasion rate of earthworms. The judgment of soil pollution is relatively vague, and it is impossible to accurately analyze the soil pollution. to determine the pollution

[0004] Through the above analysis, the existing problems and defects of the prior art are: the research of the earthworm ecotoxicity test in the prior art is relatively vague to the judgment of soil pollution, and cannot accurately determine the pollution of the soil

Images