Preparation method and application of gold nanocages

A technology of gold nano-cages and application method, which is applied in the field of preparation and application of gold nano-cages for imaging, can solve the problems of secondary transfection, cytokine storm, poor penetration, etc., and achieves good penetration and operation process. Simple, highly specific effects

Active Publication Date: 2022-05-31
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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Problems solved by technology

[0004] In view of the above problems, the present invention provides a preparation method and application of gold nanocages, which mainly solves the problem that the prior art is prone to cause cytokine storm, requires secondary transfection, has poor penetrating power, and cannot be applied to different CAR-T markers And other issues

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  • Preparation method and application of gold nanocages
  • Preparation method and application of gold nanocages
  • Preparation method and application of gold nanocages

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preparation example Construction

[0025] The preparation method of gold nanocages is as follows:

[0026] Dissolve CF3COOAg in ethylene glycol to prepare a CF3COOAg mother liquor; weigh polyvinylpyrrolidone and dissolve it in ethylene glycol to prepare a polyvinylpyrrolidone mother liquor;

[0027] Pipette concentrated hydrochloric acid and mix with ethylene glycol to prepare hydrochloric acid mother liquor; take NaHS and add it to ethylene glycol to prepare NaHS mother liquor,

[0028] Under the condition of 150℃-200℃, take NaHS mother liquor, hydrochloric acid ethylene glycol mother liquor, vinylpyrrolidone mother liquor, CF3COOAg mother liquor and react;

[0029] Transfer the reaction product obtained in the preceding steps into a centrifuge tube, add acetone solution to wash and purify, add water to disperse the obtained product, centrifuge to remove the supernatant, and wash to obtain a silver nanocube solution;

[0030] Gold nanocages were prepared by electrochemical displacement method.

[0031] The a...

Embodiment 1

[0034] 1. Preparation of silver nanocubes.

[0035] A. The CF 3 COOAg was dissolved in ethylene glycol and formulated into CF at a concentration of 100 mg / mL 3 COOAg mother liquor; Weigh 300mg of polyvinylpyrrolidone PVP (Mw=55kDa) and dissolve it in 15mL of ethylene glycol to prepare a 20mg / mL PVP mother liquor;

[0036] B. Pipette 5 μL of 37% concentrated hydrochloric acid and add it to 20 mL of ethylene glycol to prepare a hydrochloric acid mother liquor of 2.5 mmol / L; take NaHS (8.4 mg, 0.15 mmol) and add it to ethylene glycol to prepare a concentration of 10 mg / L. mL of NaHS stock solution;

[0037] C. At 150°C, mix 0.6mL of NaHS mother liquor, and after 2min, react with 5.0mL of hydrochloric acid ethylene glycol mother liquor, 12.5mL of PVP mother liquor, and 4.0mL of CF3COOAg mother liquor;

[0038] D. Transfer the reaction product obtained in step C into a centrifuge tube, add 2 to 3 times the volume of acetone solution for washing and purification, add ultrapure wa...

Embodiment 2

[0050] 1. Preparation of silver nanocubes.

[0051] A. The CF 3 COOAg was dissolved in ethylene glycol and formulated as CF at a concentration of 10 mg / mL 3 COOAg mother liquor; Weigh 300mg of polyvinylpyrrolidone PVP (Mw=55kDa) and dissolve it in 15mL of ethylene glycol to prepare a 20mg / mL PVP mother liquor;

[0052] B. Pipette 5 μL of 37% concentrated hydrochloric acid and add it to 20 mL of ethylene glycol to prepare a hydrochloric acid mother liquor of 2.5 mmol / L; take NaHS (8.4 mg, 0.15 mmol) and add it to ethylene glycol to prepare a concentration of 1 mg / L. mL of NaHS stock solution;

[0053] C. Under the condition of 150℃, mix 0.6mL of NaHS mother solution, after 2min, 5.0mL of ethylene glycol hydrochloride mother solution, 12.5mL of PVP mother solution, CF 3 COOAg mother liquor 4.0mL reaction;

[0054] D. Transfer the reaction product obtained in step C to a centrifuge tube, add 2 to 3 times the volume of acetone solution to wash and purify, then add ultrapure wa...

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Abstract

The invention belongs to the technical field of CAR-T in vivo CT imaging, and specifically discloses a preparation method and application of gold nano-cages. The preparation method of gold nanocages is to prepare CF 3 COOAg mother liquor; prepare polyvinylpyrrolidone mother liquor; prepare hydrochloric acid mother liquor; prepare NaHS mother liquor, take NaHS mother liquor, hydrochloric acid ethylene glycol mother liquor, vinylpyrrolidone mother liquor, CF 3 COOAg mother liquor reaction; transfer the reaction product obtained in the preceding steps to a centrifuge tube, add acetone solution to wash and purify, add water to disperse the product, centrifuge to remove the supernatant, and wash to obtain a silver nanocube solution; use electrochemical replacement to prepare gold nanocages. Application of gold nanocages in CAR-T in vivo CT imaging agents. The invention has the beneficial effects as follows: it is not easy to cause cytokine storm; CAR-T can be transfected at one time through electroporation, the operation process is simple, and CAR-T is imaged by CT, which has the advantages of high contrast and high specificity.

Description

technical field [0001] The invention belongs to the technical field of CAR-T in vivo CT imaging, and particularly relates to a preparation and application method of gold nano-cages for imaging. Background technique [0002] CAR-T cell therapy uses genetic engineering technology to make T cells express certain costimulatory molecules and target chimeric receptors that bind to specific tumor antigens, so as to have the ability to further activate and precisely kill specific tumor cells. Excessive dosage of CAR-T will lead to cytokine storm, and insufficient dosage will not increase the effect of leukemia treatment. Therefore, it is necessary to take effective means to monitor the in vivo behavior of CAR-T. [0003] At present, the commonly used CAR-T tracing method is reporter gene transfection. Patent 201710648782.3 discloses a method for in vivo tracing and artificial tracing of CAR-t labeling and application. This method requires the construction of lentiviral plasmids an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F9/24B22F9/16B22F1/054A61K49/04B82Y5/00B82Y30/00B82Y40/00
CPCB22F9/24B22F9/16A61K49/04B82Y5/00B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054
Inventor 胡豫梅恒唐宇翔陈钊钊姚惟琦
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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