Method for preparing citrus seed limonin by taking citrus seeds as raw material

A technology of limonin and citrus seeds, applied in the direction of steroids, organic chemistry, etc., can solve the problems of high cost, large solvent consumption, low extraction rate, etc., to ensure product quality and activity, reduce heat energy consumption, The effect of shortening the extraction time

Inactive Publication Date: 2021-02-12
哈工大机器人南昌智能制造研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for preparing citrus seed limonin from citrus seeds. Low cost, it can be applied to large-scale industrial production, and solves the problems of low extraction rate, large solvent consumption, complex process, many impurities, and high cost in previous preparation methods, which are only suitable for small-scale laboratory operations and difficult to carry out large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A method for preparing citrus seed limonin from citrus seeds, the method comprising the following steps:

[0031] (1) Pretreatment of citrus seeds: Wash fresh citrus seeds twice with water, dry them with hot air, and dry them at a temperature of 40°C for 20 hours to make the water content of citrus seeds 8%; put the dried citrus seeds into a high-speed grinder Pulverize and pass through a 40-mesh sieve to obtain citrus seed powder. The citrus seed powder is degreased with petroleum ether to extract citrus seed oil until colorless to obtain citrus seed residue. Rinse the citrus seed residue with clear water and air-dry to obtain defatted citrus seed powder. Petroleum ether can be replaced by ether, etc.;

[0032] (2) Preparation of citrus seed limonin extract: Weigh 3 kg of defatted citrus seed powder in step 1), add defatted citrus seed powder and 70% ethanol aqueous solution to a mass volume ratio of 1:8 kg / L, and add it to an ultrasonic extraction device , carry out ...

Embodiment 2

[0040] A method for preparing citrus seed limonin from citrus seeds, the method comprising the following steps:

[0041] (1) Pretreatment of citrus seeds: wash fresh citrus seeds with water for 3 times, and dry them with hot air. The temperature of hot air drying is 70°C and the drying time is 24 hours, so that the water content of citrus seeds is 8%; the dried citrus seeds are put into a high-speed grinder Pulverize and pass through a 40-mesh sieve to obtain citrus seed powder. The citrus seed powder is degreased with petroleum ether to extract the citrus seed oil until colorless to obtain citrus seed residue. The citrus seed residue is washed with water and air-dried to obtain defatted citrus seed powder;

[0042] (2) Preparation of citrus seed limonin extract: Weigh 3 kg of defatted citrus seed powder in step 1), add defatted citrus seed powder and 60% ethanol aqueous solution to a mass-volume ratio of 1:10 kg / L, and add it to an ultrasonic extraction device , carry out con...

Embodiment 3

[0050] A method for preparing citrus seed limonin from citrus seeds, the method comprising the following steps:

[0051] (1) Pretreatment of citrus seeds: Wash fresh citrus seeds twice with water, dry them with hot air, and dry them at a temperature of 50°C for 5 hours to make the water content of citrus seeds 6%; put the dried citrus seeds into a high-speed grinder Pulverize and pass through a 40-mesh sieve to obtain citrus seed powder. The citrus seed powder is degreased with petroleum ether to extract the citrus seed oil until colorless to obtain citrus seed residue. The citrus seed residue is washed with water and air-dried to obtain defatted citrus seed powder;

[0052] (2) Preparation of citrus seed limonin extract: Weigh 3 kg of defatted citrus seed powder in step 1), add defatted citrus seed powder and 80% ethanol aqueous solution to a mass volume ratio of 1:6 kg / L, and add it to an ultrasonic extraction device , carry out continuous ultrasonic extraction, the ultrason...

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PUM

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Abstract

The invention discloses a method for preparing citrus seed limonin by taking citrus seeds as a raw material. The method comprises the following steps: (1) pretreating the citrus seeds; (2) preparing acitrus seed limonin extracting solution; (3) preparing a citrus seed limonin first concentrated solution; (4) preparing a first eluent for separating and purifying the limonin from the citrus seeds;(5) preparing a citrus seed limonin second concentrated solution; (6) preparing a second eluent for secondary separation and purification of the limonin in the citrus seeds; (7) preparing a high-purity citrus seed limonin finished product. The preparation method is high in extraction rate, the prepared citrus seed limonin is high in purity, the technological process is simple, easy to operate, environmentally friendly and low in production cost, and the preparation method can be suitable for industrial large-scale production.

Description

technical field [0001] The invention relates to a method for preparing citrus seed limonin from citrus seeds. Background technique [0002] Citrus seeds are the seeds of Rutaceae Citrus plants, generally accounting for 0.1% to 4.0% of the weight of citrus fruits. Today, citrus is one of the most productive fruits in the world, with an international trade value of nearly 40 billion RMB, making it the third largest traded agricultural product. In my country's citrus processing industry, about 40,000 tons of citrus seed waste will be produced every year, but citrus seeds are not without use value. They are not only rich in oil (38.86%-42.59%), protein (28.56%-36.20%), etc. , also contains limonoid compounds, flavonoids and other bioactive ingredients, especially limonoid compounds in anti-inflammatory, analgesic, regulating cholesterol levels in the body, preventing atherosclerosis and inhibiting tumor cells, making citrus seeds It has broad prospects in the application of func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
CPCC07J73/008
Inventor 刘兆宇赵亮彭丽
Owner 哈工大机器人南昌智能制造研究院
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