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Aspergillus violaceofuscus and application thereof

A technology of Aspergillus and JX-S1, which is applied in the field of microorganisms and biological fertilizers, can solve the problems of screening and application research that have not been reported, and achieve the effects of excellent strain resources, increasing effective phosphorus content, and promoting growth and development.

Pending Publication Date: 2021-02-12
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening and application of highly efficient phosphorus-solubilizing fungal strains in the rhizosphere of Chinese fir

Method used

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  • Aspergillus violaceofuscus and application thereof
  • Aspergillus violaceofuscus and application thereof
  • Aspergillus violaceofuscus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A high-efficiency phosphorus-solubilizing fungus JX-S1 strain was screened from the rhizosphere soil of Chinese fir plantation in Shixi Forest Farm, Taihe County, Ji'an City, Jiangxi Province.

[0030] The main morphological and biological characteristics of the JX-S1 strain: cultivated on the PDA medium plate for 8 days, the colony is dark brown, has radial grooves, and has a velvety texture ( figure 1 ); the conidia are densely produced in the middle of the colony and appear dark brown; the conidia heads are radial, and the conidia are spherical or nearly spherical ( image 3 ).

[0031] figure 2 It is the phosphorus-dissolving circle produced by JX-S1 strain cultured on NBRIP medium for 7 days, indicating that JX-S1 has the ability to dissolve phosphorus.

[0032] Molecular Biology Identification:

[0033] Extract the DNA of the JX-S1 strain, use corresponding primers to PCR amplify to obtain its ITS, BenA and CaM fragments, and send them to gene sequencing. The ...

Embodiment 2

[0035] Embodiment 2: Aspergillus JX-S1 is measured at different initial pHs biomass

[0036] Inoculate the activated JX-S1 strain in PDA liquid medium (potato 200g, glucose 20g, distilled water 1000mL), 25°C, 120r / min shaking culture for 5d as seed liquid, set the pH of PDA liquid medium to 1.5, 2.5 , 3.5, 4.5, 5.5, 6.5 six gradients, divided into 250ml Erlenmeyer flasks, the filling volume is 100mL. Subsequently, the seed solution was inoculated into the PDA liquid medium according to the inoculum amount of 1% (v / v), and each treatment was repeated three times, and the culture was shaken at 25°C and 120r / min for 8 days. After the mycelia were filtered, they were dried at 65°C for 48 hours and weighed. The results are shown in Table 1. JX-S1 strains grow better under different acidic conditions of pH 1.5, 2.5, 3.5, 4.5, 5.5 and 6.5, and there is no difference in mycelial biomass. The above shows that JX-S1 strains can adapt to different pH conditions. Acidic environment, wit...

Embodiment 3

[0039] Embodiment 3: Aspergillus JX-S1 dissolves ability test to different phosphorus sources

[0040] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 2 5g, MgCl 2 5g, KCl 0.2g, MgSO 4 .7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, mix well and sterilize.

[0041] Phospholytic medium B: aluminum phosphate (AlPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly and sterilized.

[0042] Phospholytic medium C: ferric phosphate (FePO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly and sterilized.

[0043] Phosphate-dissolving medium D: calcium hydrogen phosphate (CaHPO 4 ) instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 2 , and the other ingredients and contents are the same, and they are mixed evenly and sterilized.

[0044] Phosphate-d...

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Abstract

N The invention discloses aspergillus violaceofuscus and an application thereof. The preservation number of the aspergillus violaceofuscus JX-S1 disclosed by the invention is CCTCC NO:M 2020216. The aspergillus violaceofuscus JX-S1 is screened from rhizosphere soil of a cunninghamia lanceolata man-made forest and has efficient phosphate solubilizing capacity; under different initial pH values, theJX-S1 strain has good growth and adaptability, and has a strong dissolving effect on five insoluble phosphates: calcium phosphate, iron phosphate, aluminum phosphate, calcium hydrophosphate and calcium phytate; a liquid inoculant prepared from the strain has a remarkable growth promoting effect on the ground diameter and seedling height of cunninghamia lanceolata. Therefore, an excellent strain resource is provided for developing an environment-friendly cunninghamia lanceolata biological special fertilizer.

Description

technical field [0001] The invention belongs to the field of microorganisms and biological fertilizers, and in particular relates to an aspergillus strain and its application. Background technique [0002] Chinese fir (Cunninghamia lanceolata) is the most important fast-growing and high-quality timber tree species in southern my country. It accounts for 60%-80% of the total forest area in southeastern provinces of my country. It is a timber production species with excellent economic and ecological benefits. Multi-generation continuous planting of Chinese fir will lead to a decline in forest productivity and soil fertility. In addition, in recent years, with the deforestation of Chinese fir forests and unreasonable management, the phenomenon of depletion of forest land nutrients has become increasingly serious. At present, weak soil and insufficient nutrient supply are one of the main problems in the cultivation of Chinese fir resources. For example, studies have shown that t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/34A01P21/00C12R1/66
CPCC12N1/20A01N63/34C12R2001/66C12N1/145
Inventor 万松泽张扬毛瑢陈伏生刘娟杨豆石福习张芸
Owner JIANGXI AGRICULTURAL UNIVERSITY
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