SiRNA, siRNA plasmid and lentivirus for targeted inhibition of neutral cholesteryl ester hydrolase 1 (NCEH1) gene expression, and construction methods and application of SiRNA, siRNA plasmid and lentivirus

Abstract

The invention discloses siRNA, siRNA plasmid and lentivirus for targeted inhibition of expression of neutral cholesteryl ester hydrolase 1 (NCEH1) gene of a human hepatocellular carcinoma cell, construction methods of the siRNA, the siRNA plasmid and the lentivirus, and application of the siRNA, the siRNA plasmid and the lentivirus to preparation of hepatocellular carcinoma treatment drugs for inhibiting proliferation, invasion and metastasis of the hepatocellular carcinoma cell. By researching the expression level of the NCEH1 in hepatocellular carcinoma tissue, such action target, the NCEH1gene, is provided for treatment of hepatocellular carcinoma; by designing the sequence of the siRNA for the targeted inhibition of the expression of the NCEH1 gene of the human hepatocellular carcinoma cell, the corresponding siRNA expression plasmid and siRNA lentivirus, the expression of the NCEH1 gene in the human hepatocellular carcinoma cell can be efficiently inhibited, and thus the proliferation, invasion and metastasis capability of the hepatocellular carcinoma cell is effectively inhibited; the siRNA, siRNA plasmid and lentivirus are of great significance in preparation of drugs for inhibiting proliferation, invasion and metastasis of the hepatocellular carcinoma cell so as to treat the hepatocellular carcinoma, and an effective method is provided for developing targeted therapy drugs for treating the hepatocellular carcinoma for NCEH1.

Description

technical field [0001] The present invention relates to the technical field of genetic engineering, in particular to siRNA, siRNA plasmid, lentivirus and its construction method for targeting and inhibiting the expression of NCEH1 gene in human liver cancer cells, and in the preparation of liver cancer therapeutic drugs for inhibiting the proliferation, invasion and metastasis of liver cancer cells Applications. Background technique [0002] Hepatocellular Carcinoma (HCC; liver cancer for short) now ranks fourth in the incidence of malignant tumors in my country, and its annual mortality rate has reached 20.37 / 100,000, ranking third in the mortality rate of malignant tumors in my country, seriously threatening the people of our country. life and health. Although the surgical resection rate of liver cancer has increased significantly in recent years, the treatment methods for liver cancer have become more diversified, and liver transplantation is increasingly used in the trea...

AI Technical Summary

Problems solved by technology

However, due to the short half-life of siRNA fragments, after the siRNA fragments synthesized in vitro are transferred into cells, their gene inhibition effect is generally very short-lived.

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