A total alkaloid extract and preparation method

A technology for total alkaloids and extracts, applied in the field of valerian total alkaloids extract and preparation thereof, can solve problems such as no relevant literature reports on valerian total alkaloids, and achieve high product yield and high efficacy activity Effect

Active Publication Date: 2022-06-07
武汉惠尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no relevant literature report on the extraction of total alkaloids of valerian

Method used

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  • A total alkaloid extract and preparation method
  • A total alkaloid extract and preparation method
  • A total alkaloid extract and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 Extraction process research

[0033] (1) Take valerian, decoct and extract twice with 10 times the weight of water for 1.5 hours each time, combine the secondary extracts, concentrate and dry.

[0034] (2) Take valerian, extract 2 times with 10 times the weight of 40% ethanol (containing 0.3% hydrochloric acid) for 1.5 hours each time, combine the secondary extracts, add sodium hydroxide to adjust pH to 7.5, concentrate and dry.

[0035] (3) Take valerian, extract 2 times with 10 times the weight of 60% ethanol (containing 0.3% hydrochloric acid) for 1.5 hours each time, combine the secondary extracts, add sodium hydroxide to adjust pH to 7.5, concentrate and dry.

[0036] (4) Take valerian, extract 2 times with 10 times the weight of 80% ethanol (containing 0.3% hydrochloric acid) under reflux for 1.5 hours each time, combine the secondary extracts, add sodium hydroxide to adjust pH to 7.5, concentrate and dry.

[0037] The total alkaloid content in the d...

Embodiment 2

[0043] Embodiment 2 Extraction process research

[0044] Take valerian, first use 10 times the weight of water to decoct and extract for 1.5 hours, filter, concentrate the extract into a thick paste, and then use 10 times the weight of 60% ethanol (containing 0.3% hydrochloric acid) to reflux extract the medicinal residues for 1.5 hours, filter, and then The extract is added with sodium hydroxide to adjust the pH to 7.5, then concentrated to no alcohol smell, and then added to the above thick paste and mixed.

[0045] Petroleum ether, chloroform, ethyl acetate, acetone and n-butanol are respectively added to the mixed thick paste for extraction, and the extraction sites of petroleum ether, chloroform, ethyl acetate, acetone and n-butanol are obtained after extraction, and the organic solvent is recovered. And concentrate, dry, detect the total alkaloid content in the dry paste and measure the inhibition rate to Malassezia, the results are shown in Table 2.

[0046] Table 2 Co...

Embodiment 3

[0049] Example 3 Purification process research

[0050] In order to further improve the pharmacodynamic activity of the extract, a macroporous resin purification method was used to purify the total alkaloids in the above products, as follows:

[0051] 3.1 Screening of resin types

[0052] Resin types: D101, D201, AB-8, HP-10, XAD-2, ADS-8, HPD-100.

[0053] Resin pretreatment: soak the resin in absolute ethanol for 24 hours, and then rinse with water until there is no alcohol smell.

[0054] Resin regeneration: first soak the resin with 5% hydrochloric acid for 1-3 hours and rinse with pure water until neutral, then soak with 4% sodium hydroxide for 1-3 hours, and rinse with pure water until neutral.

[0055] (1) Take 10 g of the crude extract of ethyl acetate parts, add water to dissolve, and prepare a crude extract with a concentration of 10 mg / mL.

[0056] (2) Weigh 2g of each of the above 7 kinds of macroporous resins, add them to a 100mL conical flask, add 50mL of crud...

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Abstract

The invention discloses a preparation method of valerian root total alkaloid extract, which comprises extracting valerian root with water and / or an ethanol solution containing 0.1-0.5% hydrochloric acid, extracting the extract with an organic solvent, and finally extracting the extracted part Macroporous adsorption resin purification in three steps. The invention can separate and purify the high-concentration total alkaloid extract from the valerian medicinal material, and the prepared extract has high medicinal activity.

Description

technical field [0001] The invention belongs to the field of pharmacy and relates to a valerian total alkaloid extract and a preparation method thereof. Background technique [0002] Valerian (Valeriana offcinalis L.) is a plant of the sapphire family, and it is widely distributed all over the world. Valerian has antispasmodic, analgesic, sedative and soothing effects, and can play a certain therapeutic role in bruises, nervousness, palpitations, insomnia, dysmenorrhea, amenorrhea, and rheumatic arthralgia. Valerian mainly contains volatile oils, iridoids, sesquiterpenes, alkaloids, lignans, flavonoids active ingredients, is an excellent natural medicinal resources. At present, there are not many reports on the chemical constituents and activities of valerian, mainly focusing on volatile oils, iridoids, sesquiterpenes, and lignans, and there are few studies on alkaloids. According to individual literature reports, the content of valerian alkaloids is between 1-5%, mainly i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/84A61P17/00A61P31/10
CPCA61K36/84A61P17/00A61P31/10A61K2236/333A61K2236/55
Inventor 熊义涛蔡凯曾庆奇王红芳
Owner 武汉惠尔生物科技有限公司
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