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A strain of Bacillus subtilis isolated from bee food and its application

A technology of Bacillus subtilis and bee food, which is applied in the preparation of fermented bee pollen, in the field of Bacillus subtilis, can solve the problems of restricting the development and utilization of pollen, destroying the extraction efficiency of active ingredients, etc., and achieves the promotion of digestion, absorption and release. Health benefits, the effect of increasing the content of total phenols and total flavonoids

Active Publication Date: 2022-08-09
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, bee pollen has an incomparably hard cell wall. The pollen cell wall is relatively tough, and has the physical and chemical properties of acid resistance, alkali resistance, temperature resistance, pressure resistance, and very stable to gastric acid and other digestive system enzymes. When the pollen directly enters the human digestive tract, Only a small part of nutrients can be released through the germination pores on the pollen surface, and the existence of the pollen wall limits the development and utilization of pollen
However, if the bee pollen is extracted by high temperature and high pressure, it is easy to destroy the active ingredients and the extraction efficiency is very low.

Method used

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  • A strain of Bacillus subtilis isolated from bee food and its application
  • A strain of Bacillus subtilis isolated from bee food and its application
  • A strain of Bacillus subtilis isolated from bee food and its application

Examples

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Effect test

Embodiment 1

[0023] Example 1 Separation, purification and identification of microorganisms

[0024] 1. Isolation and purification of microorganisms in bee food

[0025] Aseptic operation, take 5g of bee food, put it in a 150ml sterile conical flask, add 45ml sterile water, shake and mix well, and then dilute to an appropriate concentration. Draw 100 μl of the diluted sample solution of each concentration into LB solid medium, spread the bacterial liquid evenly with a coater, and cultivate in a constant temperature incubator at 37°C for 20 hours.

[0026] Single colonies with different shapes were picked, streak-inoculated on LB solid plates, and cultured in a constant temperature incubator at 37°C for 20h. Repeat the above operation and purify for 4-5 generations until pure colonies are obtained. The resulting single strains were stored at -80°C in 50% LB liquid medium and 50% glycerol tubes containing 40% glycerol.

[0027] 2. Strain identification

[0028] 2.1 Observation of colony ...

Embodiment 2

[0055] The application of embodiment 2 microorganisms in fermented bee pollen

[0056] 1. Bacteria culture

[0057] The two strains of bacillus obtained by the above separation and purification and another strain of bacillus licheniformis preserved in the laboratory were cultured respectively, and the method was as follows:

[0058] (1) Bacillus licheniformis: Pick 2 rings of Bacillus licheniformis from the solid plate, insert it into a 250ml Erlenmeyer flask with 30ml LB medium, 37°C, 120r / min shaking culture for 12h, and then inoculate with 10% The amount was transferred into a 250ml Erlenmeyer flask containing 30ml of LB medium, and incubated at 37°C with shaking at 120r / min for 10h.

[0059] (2) Bacillus subtilis: Pick 2 rings of Bacillus subtilis from the solid plate separated and purified above, insert into a 250ml Erlenmeyer flask containing 30ml LB medium, 37 ° C, 120r / min shaking culture for 12h, then with 10% of the inoculum was placed in a 250ml Erlenmeyer flask c...

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Abstract

The present invention disclosed a Bacillus Subtilis XMFFL separated from the bee grain. It is located in the typical cultivation center of China, and the preservation number is CCTCC NO: M 2020782.The strain has a good fermentation ability for bee pollen, which can transform and form more total phenols and total flavonoids.The present invention also revealed a method of fermentation bee pollen. This method can increase the total phenol content of the bee pollen to 2.19 times, and the total flavonoid content is increased to 1.72 times, which gives the bee pollen better health effect.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, relates to a Bacillus subtilis isolated from bee grains, and also relates to the application of the Bacillus subtilis in preparing fermented bee pollen. Background technique [0002] Bee food, also known as hive pollen and bee bread, is not only food for young and worker bees, but also a source of nutrition for larval development. Many species of the superfamily of bees have the habit of brewing bee food. Take bees as an example. Worker bees collect pollen and unload them in the hive, and back-end bees chew and mix honey and compact them. Pollen, bee secretions and honey are mixed into a chyle-shaped pollen mass, which is fermented and brewed into peak food by the action of microorganisms in a beehive at a certain temperature and humidity. The microbial fermentation and brewing of pollen mass is the key to the formation of bee food, and it is also a determinant factor affecting the quality ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L21/20A23L33/00C12R1/125
CPCA23L21/20A23L33/00A23V2002/00A23V2200/30Y02P60/87
Inventor 刘睿张会芳唐翠娥鲁群
Owner HUAZHONG AGRI UNIV
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