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A method for extracting and separating baicalin and baicalein

A technology of baicalein and baicalin, which is applied in the field of traditional Chinese medicine extraction and achieves the effects of realizing recycling, short operation time and simple method

Active Publication Date: 2022-04-08
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Imidazole-based ionic liquids refer to a class of ionic liquids with different cationic alkyl substituents containing imidazole groups. They have the advantages of easy preparation and easy structure regulation, and are currently one of the most studied ionic liquids. The method of baicalin and baicalein mainly uses imidazole ionic liquid bromide to extract baicalin, but it is mainly used for the extraction of baicalin. At present, there is no research and research on the simultaneous extraction and separation of baicalin and baicalein from baicalin flavonoids. record

Method used

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  • A method for extracting and separating baicalin and baicalein
  • A method for extracting and separating baicalin and baicalein
  • A method for extracting and separating baicalin and baicalein

Examples

Experimental program
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Effect test

Embodiment 1-5

[0031] The method of above-mentioned step S1 is used to extract and separate baicalin and baicalein in Scutellaria baicalensis. In Examples 1-5, other extraction conditions are the same, and are respectively: solid-liquid ratio 1g:60mL, extraction temperature 60°C, extraction time 45min, The ultrasonic time is 5min, the difference is that the volume ratio of the two phases of the extraction agent is different, and after the extraction, the extraction rate and the partition coefficient of baicalin and baicalein are tested respectively, as shown in Table 1:

[0032] Selection and extraction effect of different two-phase volume ratios in table 1 extractant

[0033]

Embodiment 6-10

[0035] In embodiment 6-10, the method of above-mentioned step S1 is used for extraction, wherein, other conditions are: two volume ratio is 1:1, extraction temperature is 60 ℃, extraction time is 45min, ultrasonic time is 5min, difference is, Different solid-liquid ratios were selected for extraction, as shown in Table 2:

[0036] Table 2 Selection and extraction effect of different solid-liquid ratios

[0037]

[0038]

Embodiment 11-15

[0040] In Examples 11-15, the method of the above step S1 was used for extraction, wherein the other conditions were: the volume ratio of the two phases was 1:1, the solid-liquid ratio was 1g:40mL, the extraction time was 45min, and the ultrasonic time was 5min, The difference is that the extraction temperature is different, as shown in Table 3:

[0041] Table 3 Selection of different extraction temperatures and extraction effects

[0042]

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Abstract

The invention provides a method for extracting and separating baicalin and baicalein, comprising the following steps: using 1-ethyl-3-methylimidazolium hexafluorophosphate ionic liquid and water to form a two-phase system as an extractant, extract the traditional Chinese medicine Scutellaria baicalensis; the extraction temperature is 60-80°C; after the extraction is completed, centrifuge and separate the two phases to obtain an aqueous phase rich in baicalin and an ionic liquid phase rich in baicalein; the ionic liquid obtained in step S2 Add methanol to the phase to form a mixed solution, dissolve baicalein in methanol, then precipitate the ionic liquid, and separate the solid and liquid; Extracting and separating the baicalin and baicalein can effectively extract and separate and recover the baicalin and baicalein; moreover, the method of the invention is simple and the operation time is short.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, and more particularly, to a method for extracting and separating baicalin and baicalein. Background technique [0002] Scutellaria baicalensis is a perennial herbaceous plant of the genus Scutellaria baicalensis. It contains a variety of biologically active ingredients. Its root can be used as medicine, which has the functions of clearing away heat and dampness, purging fire and detoxifying, lowering blood pressure, hemostasis, and tocolysis. The chemical components in Scutellaria baicalensis mainly include flavonoids, phenylethanoid glycosides, volatile oils, sterols, alkaloids, amino acids and trace elements. Hebaicalein belongs to flavonoids and is the main active ingredient in traditional Chinese medicine Scutellaria baicalensis. Since flavonoids are insoluble in water, traditional methods for separating baicalin and baicalein from scutellaria mainly use organ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40C07H17/07C07H1/08B01D11/02
CPCC07D311/30C07D311/40C07H17/07C07H1/08B01D11/0265
Inventor 谭志坚易永健王玲琪余旺曾粮斌王朝云汪洪鹰杨媛茹
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI