Reagent for detecting SNP molecular marker combination related to anti-nervous necrosis virus character of epinephelus

A nerve necrosis and molecular marker technology, applied in the application field of China, can solve the problems of economic loss, great harm, and no effective treatment for the disease, and achieve the effect of speeding up the cultivation process, improving the ability, and speeding up the cultivation process.

Active Publication Date: 2021-03-16
SOUTH CHINA AGRI UNIV
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Red-spotted grouper nervous necrosis virus (RGNNV) is very harmful to adults and juveniles of grouper, serious When the mortality rate can reach 100%, it often causes huge economic losses to the grouper aquaculture and seriously threatens the healthy development of the grouper aquaculture industry. However, there is still no effective treatment for the disease caused by the virus at present. monitoring
[0004] Chinese patent CN201610255794.5 discloses a method for detecting grouper neuronecrosis virus infection based on nucleic acid aptamer-based Sandwich ELASA; CN201610465941.1 discloses a Coll

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent for detecting SNP molecular marker combination related to anti-nervous necrosis virus character of epinephelus
  • Reagent for detecting SNP molecular marker combination related to anti-nervous necrosis virus character of epinephelus
  • Reagent for detecting SNP molecular marker combination related to anti-nervous necrosis virus character of epinephelus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0089] Example 2 PCR-sequencing verification analysis of SNPs loci associated with neuronecrosis virus resistance traits in red-spotted grouper

[0090] 1. Experimental method

[0091] 1) Primer design

[0092] According to the position information of 5 SNP sites obtained in Example 1, the upstream and downstream sequences of these SNP sites are called in the grouper genome database, and the sequence information is as follows: figure 1 Display, and design SNP site primers based on these sequence information, the primer information is as follows:

[0093] SNP-1:

[0094] Upstream primer F: 5'-CCTGCTCACTGGACCCTCAC-3' (SEQ ID NO.6);

[0095] Downstream primer R: 5'-CAGTCCCAAGCCACGAGAATA-3' (SEQ ID NO.7).

[0096] SNP-2:

[0097] Upstream primer F: 5'-CTGCCCGTCTGGGAAACTCT-3' (SEQ ID NO.8);

[0098] Downstream primer R: 5'-AGGAAATCGGCTCTGGTGTT-3' (SEQ ID NO.9).

[0099] SNP-3:

[0100] Upstream primer F: 5'-CACTTCCCTGCTGTCCTTTG-3' (SEQ ID NO.10);

[0101] Downstream primer...

Embodiment 3

[0121] Example 3 A method for detecting the resistance of red-spotted grouper to neuronecrosis virus

[0122] By detecting the five SNP sites in Example 2 of the red-spotted grouper sample to be tested, the disease resistance of the red-spotted grouper to be tested is determined.

[0123] Include the following steps:

[0124] (1) extract the genomic DNA of the red-spotted grouper to be tested;

[0125] (2) Using the DNA obtained in step (1) as a template and the nucleotide sequences shown in SEQ ID NO.6-7, 8-9, 10-11, 12-13 and 14-15 as primers, respectively PCR amplification to obtain PCR amplification products;

[0126] (3) Sequencing the PCR amplification product obtained in step (2), and determining the genotypes of SNP sites 1 to 5 of the red-spotted grouper to be tested;

[0127] (4) Determine whether the red-spotted grouper to be tested is resistant to nerve necrosis virus according to the genotype of the SNP sites 1 to 5 determined in step (3):

[0128] SNP site 1 ...

Embodiment 4

[0133] Example 4 A kit for detecting the resistance of red-spotted grouper to neuronecrosis virus

[0134] 1. Composition

[0135] The nucleotide sequence is shown as primers and PCR reagents shown in SEQ ID NO.6-15.

[0136] 2. How to use

[0137] With embodiment 3.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a reagent for detecting an SNP molecular marker combination related to the anti-nervous necrosis virus character of epinephelus. Five SNP sites related to the anti-nervous necrosis virus character of epinephelus akaara are found; and when the genotype of the SNP site 1 is GG, the genotype of the SNP site 2 is AC, the genotype of the SNP site 3 is CC, the genotype of the SNPsite 4 is TA and the genotype of the SNP site 5 is TT, the death probability of the epinephelus akaara infected with nervous necrosis virus is obviously lower than that of individuals with other genotypes. By detecting the SNPs of the epinephelus akaara, whether the epinephelus akaara has the anti-nervous necrosis virus character or not can be effectively determined.

Description

technical field [0001] The present invention relates to the application in. The technical field, in particular, relates to a reagent for detecting the combination of SNP molecular markers related to the anti-necrosis virus trait of grouper. Background technique [0002] Red-spotted grouper (Epinephelus akaara), commonly known as red spot, belongs to the genus Epinephelus of the family Serranidae in the order Perciformes. It is mainly distributed in the western part of the North Pacific Ocean. It is produced in the south of the Zhoushan Islands in China and through the Taiwan Strait to the South China Sea. , Beibu Gulf area. It has the characteristics of delicious meat and high nutritional value, and is an important grouper breeding species in the coastal areas of South China. [0003] Red-spotted grouper nerve necrosis virus (red-spotted grouper nervous necrosis virus, RGNNV) is very harmful to adults and juveniles of grouper, and the mortality rate can reach 100% in sever...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/686C12Q2600/156C12Q2600/124Y02A50/30
Inventor 杨敏秦启伟王庆陈锦鹏李鑫帅黄健玲
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap