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Bladder cancer organoid culture medium and culture method

A culture method and organoid technology, applied in the field of bladder cancer organoid culture medium and its culture, can solve the problems of slow growth, low culture success rate, and less organ growth

Pending Publication Date: 2021-03-19
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
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  • Application Information

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Problems solved by technology

[0014] The purpose of the present invention is to provide a method for culturing bladder cancer organoids, which can improve the success rate of bladder cancer organoids and the number of passages to solve the problem of less growth of bladder cancer organoids in the prior art and relatively poor cell morphology. Poor, and slow growth and other issues
[0015] Another object of the present invention is to provide a culture medium for bladder cancer organoids. The medium culture method can improve the success rate of bladder cancer organoids and the number of passages to solve the problem of the culture of bladder cancer organoids in the prior art. Problems such as low success rate and low number of passages

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  • Bladder cancer organoid culture medium and culture method
  • Bladder cancer organoid culture medium and culture method

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Embodiment Construction

[0017] The invention provides a method for culturing bladder cancer organoids.

[0018] A method for culturing bladder cancer organoids, comprising the following steps:

[0019] A) Take the bladder cancer tissue block, cut it into pieces, add the first digestive solution to digest, centrifuge to obtain the first precipitate, digest the first precipitate with the second digestive solution, centrifuge to obtain the second precipitate, and neutralize the culture medium Resuspend the second precipitate, filter to remove large pieces of tissue, and centrifuge the filtrate to obtain the third precipitate;

[0020] B) under ice-bath conditions, resuspend the third precipitate with organoid medium A and Matrigel, inoculate, and incubate;

[0021] C) After the matrigel inoculated in step B) is solidified, organoid medium A is added, cultured for 3 days, and then replaced with organoid medium B for 7-12 days to obtain bladder cancer organoids.

[0022] The filtration was performed wit...

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Abstract

The invention provides a bladder cancer organoid culture medium and a culture method. According to the culture method, two different organoid culture mediums are adopted for culture, and the composition and proportion of the two organoid culture mediums are disclosed. According to the method and the culture medium, the culture success rate, the passage frequency, the growth speed and the growth form of the bladder cancer organoid can be improved, and the problems that in the prior art, the culture success rate is low, the passage frequency is small, the organoid grows less, the cell form is poor, and growth is slow of the bladder cancer organoid are solved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a culture medium and its application, and more specifically to a bladder cancer organoid culture medium and a culture method thereof. Background technique [0002] Tumor organoids, also known as "cancer substitutes" and "tumor-like" etc., are mainly used for in vitro 3D culture of tumor tissues from patients to simulate the biological characteristics of tumor tissues in vivo. Organoids derived from precancerous lesions (such as intraepithelial neoplasia) are mainly used to simulate tumor development and analyze tumor-related omics changes. Tumor organoids highly summarize the characteristics of the source tumor tissue and retain the heterogeneity between individuals, so they have application value in translational medicine and can be used for functional tests such as high-throughput drug screening and even individualized treatment plans formulation. [0003] Bladder cancer ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09
CPCC12N5/0693C12N5/0697C12N2513/00C12N2501/999C12N2501/998
Inventor 黄卫人余磊陈巍李志超
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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