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Key Regulatory Genes of Tomato Irregular Fruit Cracking and Their Identification Methods and Applications

A technology that regulates genes and irregularities, and is used in applications, genetic engineering, plant genetic improvement, etc.

Active Publication Date: 2022-04-19
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In tomato, EXP, PG, and TBG6, which affect cell wall degradation, have been found to affect tomato fruit dehiscence, but were found to have less effect on fruit dehiscence through transgenics (Moctezuma et al., 2003; Jiang et al., 2019), possibly Not the major gene regulating fruit dehiscence

Method used

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  • Key Regulatory Genes of Tomato Irregular Fruit Cracking and Their Identification Methods and Applications
  • Key Regulatory Genes of Tomato Irregular Fruit Cracking and Their Identification Methods and Applications
  • Key Regulatory Genes of Tomato Irregular Fruit Cracking and Their Identification Methods and Applications

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Materials and methods

[0032] 1.1. Test materials and F 2 Group construction

[0033] (1) Through the investigation on the fruit cracking rate of more than 600 tomato materials for two consecutive years in the early stage, the easy cracking fruit germplasm S189 with stable fruit cracking characteristics (quoted from the Tomato Genetic Resource Center (TGRC), numbered LA2661) and the cracking resistant germplasm R91 were screened out. (Quoted from Tomato Cherry in the United States), the average fruit cracking rate of S189 red ripening period is 91.02%, and the average fruit cracking rate of R91 red ripening period is 9.25%. These two lines have been self-crossed for more than 6 generations, and the fruit cracking traits are stable.

[0034] (2) The female parent of the tomato cracking-resistant germplasm 'R91' and the easy-cracking germplasm 'S189' were used as the male parent to obtain F 1 generation, self-breeding preparation F 2 generational segregation groups...

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Abstract

The invention discloses a key regulation gene of tomato irregular fruit cracking, an identification method and application thereof. The present invention identifies for the first time the key gene regulating tomato irregular fruit cracking Solyc02g076780 , which is related to the signal transduction pathway of ethylene-responsive protein kinase Le‑CTR1, and should be an upstream gene regulating fruit cracking. This study can lay a theoretical foundation for the directed cultivation of cracking-resistant tomato varieties using modern molecular biology and biotechnology, and can provide genetic resources for the breeding of cracking-resistant varieties, so it has important scientific significance and application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a key regulation gene of tomato irregular fruit cracking, an identification method and application thereof. Background technique [0002] Fruit cracking is a common and urgent problem in the production of fruit horticultural crops. Irregular fruit cracking of tomato is a common way of fruit cracking. After the fruit cracks, it will affect its appearance quality, shorten the shelf life, and even be infected with mold, which will reduce or even lose the commercial value of the fruit, which greatly hinders the production and sales of the fruit. In view of the extensiveness and seriousness of the fruit cracking problem, how to reduce the fruit cracking has become an urgent problem to be solved. In production practice, fruit cracking can be alleviated to a certain extent by balancing fertilizer and water, spraying hormones or applying mineral elements, but clarifying the mech...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/82C12Q1/6895A01H5/08A01H6/82
CPCC07K14/415C12N15/8261C12Q1/6895C12Q2600/124C12Q2600/156
Inventor 蒋芳玲陈斌吴震刘东波周怀强林昊维罗国舟
Owner NANJING AGRICULTURAL UNIVERSITY