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Cold region straw decomposing inoculant as well as preparation method and application thereof

The technology of bacteriolysis agent and straw is applied in the field of agricultural preparations, which can solve the problems of high labor and economic cost, and achieve the effects of improving and maintaining enzyme activity, protecting bacterial activity and reducing damage.

Active Publication Date: 2021-03-30
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual use of this bacterial agent, the human and economic costs such as transportation and spraying are relatively high.

Method used

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  • Cold region straw decomposing inoculant as well as preparation method and application thereof
  • Cold region straw decomposing inoculant as well as preparation method and application thereof
  • Cold region straw decomposing inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The invention relates to a straw decomposing bacterial agent in cold regions, which consists of microcapsules of low-temperature bacterial flora and bacteria powder of normal-temperature lignocellulose-decomposing flora.

[0049] (1) Production of low-temperature flora microcapsules:

[0050] ①Preparation of improved low-temperature bacterial culture medium: the preparation contains glucose 15g / L, K 2 HPO 4 1.0 g / L, KH 2 PO 4 1.0g / L, peptone 6g / L, beef extract 6g / L, yeast powder 3g / L, MgSO 4 ·7H 2 O0.1g / L, sodium acetate 2g / L, diammonium hydrogen citrate 2g / L, MnSO 4 ·H 2 O 0.05g / L, Tween 801mL / L improved low-temperature bacterial culture medium. Divide into 500mL blue cap bottles, each bottle contains 350mL. Store in a 4°C refrigerator for later use.

[0051] ②Floral culture: Inoculate Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Lactococcus, Arthrobacter globosa and Rhodococcus into the improved low-temperature culture medium. After culturing a...

Embodiment 2

[0061] The invention relates to a straw decomposing bacterial agent in cold regions, which is composed of: freeze-dried powder of low-temperature bacterial flora and bacterial powder of normal-temperature lignocellulose-decomposing bacterial flora.

[0062] (1) Production of low-temperature flora freeze-dried powder:

[0063] ①Preparation of improved low-temperature bacterial culture medium: the preparation contains glucose 15g / L, K 2 HPO 4 1.0 g / L, KH 2 PO 4 1.0g / L, peptone 6g / L, beef extract 6g / L, yeast powder 3g / L, MgSO 4 ·7H 2 O0.1g / L, sodium acetate 2g / L, diammonium hydrogen citrate 2g / L, MnSO 4 ·H 2 O 0.05g / L, Tween 801mL / L improved low-temperature bacterial culture medium. Divide into 500mL blue cap bottles, each bottle contains 350mL. Store in a 4°C refrigerator for later use.

[0064] ②Floral culture: Inoculate Lactobacillus plantarum, Lactococcus lactis subsp. lactis, Lactococcus, Arthrobacter globosa and Rhodococcus into the improved low-temperature culture...

Embodiment 3

[0070] The bacterial agent prepared in Example 1 was used as the experimental group, and was simultaneously inoculated with four commercially available straw-decomposing bacterial agents CK1, CK2, CK3, and CK4 on a medium with rice straw as the only carbon source, and fermented at 28°C for 15 days , to determine the weight-reducing ability of rice straw. Under different treatments, the analysis of rice straw weight loss is as follows: figure 1 shown. Depend on figure 1 It can be seen that the straw weight loss under the treatment of the bacterial agent, CK1, CK2, CK3, and CK4 was 0.72g, 0.67g, 0.63g, 0.58g, and 0.54g, and the weight loss rates were 44.2%, 41.10%, and 38.65% respectively. %, 35.58%, 33.12%. It shows that the straw transformation ability of this inoculum agent under the same conditions is stronger than that of the four commercially available inoculum agents used in the experiment.

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Abstract

The invention provides a cold region straw decomposition microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of agricultural preparations. The cold region straw decomposition microbial inoculum comprises a low-temperature flora and a normal-temperature flora, wherein the low-temperature flora comprises 20%-28% of lactobacillus plantarum, 17%-25% of lactococcus lactis subsp. Lactis, 14%-22% of lactococcus, 11%-22% of arthrobacter globiformis and 9%-18% of rhodococcus, wherein the normal-temperature flora is lignocellulose decompositionflora. The product provided by the invention can start fermentation under a low-temperature condition, promote decomposition of straws under the low-temperature condition, improve the in-situ decomposition efficiency of straws in cultivated land, and solve the problems of seedling death and yield reduction caused by severe decomposition of straws in paddy fields in cold regions in summer in China.

Description

technical field [0001] The invention belongs to the technical field of agricultural preparations, and in particular relates to a straw decomposing bacterial agent in cold regions, a preparation method and application thereof. Background technique [0002] Straw is the most productive renewable resource in the world. According to incomplete statistics, the annual output of straw in the world is 85 billion tons, and the annual output of crop straw in my country alone reaches 850 million tons. Lignocellulose in straw is combined by cellulose, hemicellulose, lignin, ash and other substances, which makes cellulose difficult to be hydrolyzed, so it is compared to "concrete". There is a huge amount of straw in the main grain-producing areas of Northeast China. With the sharp decline in the marginal effect of the investment ratio of cultivated land, the degraded black soil urgently needs to be fertilized, and there is an urgent need for straw returning to the field to improve the e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C12N1/14C12N1/04C12N11/10C12N11/04C05F11/08C05F17/20C12R1/25C12R1/06C12R1/01C12R1/69C12R1/80C12R1/885C12R1/125C12R1/145C12R1/085C12R1/865C12R1/84
CPCC05F11/08C12N1/04C12N1/14C12N1/16C12N1/18C12N1/20C12N11/04C12N11/10C05F17/20Y02W30/40
Inventor 刘长莉
Owner NORTHEAST FORESTRY UNIVERSITY
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