A kind of preparation method of restorer line japonica rice at late flowering time

Abstract

The invention provides a method for preparing a restorer japonica rice at late flowering time. The preparation method comprises: planting the seeds of the first generation of backcross progeny to obtain the second generation of progeny plants; After 11:00 and the plant with the restorer gene Rf1a is backcrossed with the japonica rice to obtain the second-generation seed of the backcross; plant the second-generation seed of the backcross to obtain the second-generation plant of the backcross; Among the second-generation plants of the backcross, select the plants with flowering time after 11:00 in the morning and have the restorer gene Rf1a and cultivate them to obtain the first-generation self-cross seeds; add the first-generation self-cross seeds until the properties are stable, A late-flowering restorer line of japonica rice was obtained. The late-flowering restorer japonica rice obtained by the preparation method has a late flowering time and can improve the pollination and seed-setting rate of the hybrid japonica rice.

Description

technical field [0001] The invention relates to the field of plant breeding, more specifically, to a preparation method of late flowering restorer line japonica rice. Background technique [0002] Rice is one of the most important food crops in the world. More than half of the world's population uses rice as a staple food. Therefore, rice production is related to world food security. The use of heterosis is an effective way to increase rice yield. Great achievements have been made in the development of hybrid indica rice in China, but the development of hybrid japonica rice is still relatively slow. [0003] Low seed yield is an important bottleneck restricting the development of hybrid japonica rice. At present, the japonica CMS line and the indica-japonica restorer line are used as parents to prepare hybrid japonica rice, but the failure of the parents to flower is the key factor leading to the low seed yield of hybrid japonica rice. Specifically, indica rice flowered ea...

AI Technical Summary

Problems solved by technology

At present, the japonica CMS line and the indica-japonica restorer line are used as parents to prepare hybrid japonica rice, but the failure of the parents to flower is the key factor leading to the low seed yield of hybrid japonica rice

Specifically, indica rice flowered early, japonica rice flowered late, and the japonica rice CMS was directly transformed from japonica rice, coupled with the sterile cytoplasmic effect, resulting in late flowering, usually after 11:00; while indica-japonica cross The restorer line flowered early, usually between 10:00 and 11:30, that is, after the indica-japonica restorer line flowered and scattered powder, the japonica rice CMS line flowered belatedly, which made the indica-japonica restorer line different from the japonica rice. The flowering time of the breeding lines only overlaps for half an hour. Crossing the indica-japonica restorer line and the japonica rice male sterile line as parents will result in a low pollination and seed setting rate on the flowering day of the japonica rice male sterile line, and the general pollination and seed setting rate is about 20%, resulting in Low seed yield restricts the development of hybrid japonica rice

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