Application of substance for detecting genetic marker to preparation of kit for risk early warning and early diagnosis of rectal cancer

A technology of genetic markers and risk early warning, which is applied to the detection of genetic markers in the field of preparation of risk early warning and early diagnosis of rectal cancer kits, which can solve problems such as delayed treatment and unobvious early symptoms of sCRC

Inactive Publication Date: 2021-04-02
NORTHWEST UNIV(CN)
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Problems solved by technology

[0005] The problem existing in the existing technology is that because the early symptoms of sCRC ...

Method used

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  • Application of substance for detecting genetic marker to preparation of kit for risk early warning and early diagnosis of rectal cancer
  • Application of substance for detecting genetic marker to preparation of kit for risk early warning and early diagnosis of rectal cancer
  • Application of substance for detecting genetic marker to preparation of kit for risk early warning and early diagnosis of rectal cancer

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Embodiment 1

[0026] (1) Collection and preparation of clinical samples

[0027] According to clinical diagnosis and physical examination results, tumor tissues and normal tissues of 150 sCRC patients in the Chinese Han population were collected, of which 3 were frozen fresh tissues and their own blood samples for exome sequencing in the early stage, and the rest were paraffin-embedded samples for later stage Independent sample research, detailed records of sample clinical and pathological information. Inclusion criteria for the disease group: sCRC diagnosed by immunohistochemical detection (must include pathological diagnosis) for the first time, no obvious family medical history, and detection of mutation types of MLH1 and MSH2 genes that have diagnostic significance for the confirmed hereditary CRC of Chinese Han people Negative results; tumor tissue was stained by HE and confirmed that the proportion of tumor cells was greater than 80%; normal tissue refers to tissue with no tumor cells...

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Abstract

The invention belongs to the technical field of biomedicine, and particularly relates to an application of a substance for detecting a genetic marker to preparation of a kit for risk early warning andearly diagnosis of rectal cancer. Tumor tissue samples of three Chinese Han family sCRC patients are sequenced and analyzed by utilizing an exon group sequencing technology, and specific low-frequency function deletion mutation of screened tumor tissues is determined by genotyping large-sample tumor tissues and normal control tissues of the large-sample tumor tissues. Finally, functional geneticmarkers of new candidate genes BMP5, RASSF6, DDI2 and SARDH which can be used for early-stage sCRC risk early warning.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the use of a substance for detecting genetic markers in the preparation of a risk warning and early diagnosis kit for rectal cancer. Background technique [0002] Sporadic colorectal cancer (sCRC) is a type of malignant tumor originating from the mucosal epithelium of the large intestine without obvious family genetic predisposition and family history, and its prevalence accounts for about 70% of colorectal cancer (CRC). %. According to the "2010 China Health Statistical Yearbook" of the Ministry of Health of China, malignant tumors have leapt to the top of the death rate of major diseases in my country, among which sCRC is one of the five most harmful malignant tumors in China, and the mortality rate of urban residents is as high as 9.78 / 100,000 . With the development of my country's economy and the transformation of people's lifestyle, the incidence and mortali...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 杨进陈尔飞燕变变
Owner NORTHWEST UNIV(CN)
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