Light guide plate, fluorescence detection optical system and large-batch nucleic acid detection method

Abstract

The invention discloses a light guide plate, a fluorescence detection optical system and a large-batch nucleic acid detection method, and belong to the field of molecular diagnosis detection instruments. The light guide plate comprises a light guide plate body, a groove group is arranged on the upper surface of the light guide plate body, and the groove group comprises a plurality of grooves arranged in parallel; the length direction of each groove is marked as the X direction, the width direction of each groove is marked as the Y direction, and the grooves arranged in one column in the Y direction form a groove group; and the depth of the groove is smaller than the thickness of the light guide plate. The light guide plate, the fluorescence detection optical system and the large-batch nucleic acid detection method can effectively solve the problem of nonuniformity of light beams.

Description

technical field [0001] The present invention relates to the fields of medical inspection and testing instruments and molecular diagnostic testing instruments, and more specifically, relates to a light guide plate, a fluorescence detection optical system, and a large-scale nucleic acid detection method. Background technique [0002] Multi-sample fluorescence detection optical system is a development direction of molecular diagnostic detection equipment. [0003] Regarding the multi-sample fluorescence detection optical system, the existing technologies are as follows: [0004] Shanghai Lanyi Technology Co., Ltd. discloses a multi-sample microbial detection device in CN104964927A, which simultaneously completes the multi-parameter detection of colorimetry, turbidimetry and fluorescence of a matrix sample hole by scanning, and can simultaneously detect 16 samples to be tested . The detection method of the detection device of the invention is simple and efficient, and has good...

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Problems solved by technology

[0014] On the same day, the application for "A Fluorescence Detection Optical System with Single Light Source and Multiple Samples and Its Working Method" protected the fluorescence detection method, but one of the core components of the scheme is the light guide plate, which is a successful optical system for the fluorescence detection of single light source and multiple samples. The core key to whether or not, that is, the uniformity of the mother beam directed to the reaction cell assembly is the core that affects the test results. If its uniformity cannot meet the requirements, it will lead to misjudgment of the detection

[0015] However, after searching databases such as Himmpat, CNKI, and EPO, the inventor team found that there is no relevant information on how to design light guide plates in the prior art. In particular, related issues have not been reported.

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