A light guide plate, a fluorescence detection optical system, and a large-scale nucleic acid detection method
A technology of light guide plate and groove, which is used in the field of fluorescence detection optical system, light guide plate, and large-scale nucleic acid detection, can solve the problems such as how to design the light guide plate, the related problems have not been reported, and the uniformity cannot meet the requirements.
Active Publication Date: 2021-10-08
SUZHOU MOLARRAY CO LTD
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Problems solved by technology
[0014] On the same day, the application for "A Fluorescence Detection Optical System with Single Light Source and Multiple Samples and Its Working Method" protected the fluorescence detection method, but one of the core components of the scheme is the light guide plate, which is a successful optical system for the fluorescence detection of single light source and multiple samples. The core key to whether or not, that is, the uniformity of the mother beam directed to the reaction cell assembly is the core that affects the test results. If its uniformity cannot meet the requirements, it will lead to misjudgment of the detection
[0015] However, after searching databases such as Himmpat, CNKI, and EPO, the inventor team found that there is no relevant information on how to design light guide plates in the prior art. In particular, related issues have not been reported.
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Embodiment 1
[0099] Embodiment one, such as figure 1 As shown, a fluorescence detection optical system with a single light source and multiple samples includes: a light source emitting component 100, a light source receiving component 200, and a light guide-reaction component 300;
[0100] Wherein, the light source emitting assembly 100 includes: LED lamp 101 (using LED as the light source, the service life can reach 20,000 hours, of course, it is also feasible to use other monochromatic light sources, such as: halogen tungsten lamp), the first convex lens 102 (spherical mirror), the second Two convex lenses 103 (spherical mirror), excitation filter 104, light guide strip assembly 105; the LED lamp faces downward, the convex surface of the first convex lens 102 faces downward, and the second convex lens 103 is arranged on the first Below the convex lens 102 and the convex surface of the second convex lens 103 faces downward;
[0101] The upper surface of the second convex lens is circula...
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The invention discloses a light guide plate, a fluorescence detection optical system, and a large-scale nucleic acid detection method, which belong to the field of molecular diagnostic detection instruments. Groove group, the groove group includes several grooves arranged in parallel, the length direction of the grooves is marked as the X direction, the width direction is marked as the Y direction, and a plurality of grooves arranged in a row along the Y direction The grooves together form a groove group; the depth of the grooves is smaller than the thickness of the light guide plate. The present invention aims to provide a light guide plate, a fluorescence detection optical system, and a large-volume nucleic acid detection method, which can effectively solve the inhomogeneity of light beams.
Description
technical field [0001] The present invention relates to the fields of medical inspection and testing instruments and molecular diagnostic testing instruments, and more specifically, relates to a light guide plate, a fluorescence detection optical system, and a large-scale nucleic acid detection method. Background technique [0002] Multi-sample fluorescence detection optical system is a development direction of molecular diagnostic detection equipment. [0003] Regarding the multi-sample fluorescence detection optical system, the existing technologies are as follows: [0004] Shanghai Lanyi Technology Co., Ltd. discloses a multi-sample microbial detection device in CN104964927A, which simultaneously completes the multi-parameter detection of colorimetry, turbidimetry and fluorescence of a matrix sample hole by scanning, and can simultaneously detect 16 samples to be tested . The detection method of the detection device of the invention is simple and efficient, and has good...
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IPC IPC(8): G02B6/00G01J3/10G01J3/12G01J3/44G01N21/64
CPCG02B6/0016G02B6/0038G01J3/4406G01J3/10G01J3/12G01N21/64G01N21/645
Inventor 李晓刚李慧王威梅宗羲王野聂晶
Owner SUZHOU MOLARRAY CO LTD