Accurate and rapid detection method for pesticide residues in fruits

A technology for pesticide residues and detection methods, applied in the detection field, can solve the problems of high cost, lengthy time, low recovery rate, etc., and achieve the effects of reducing processing time and cost, being easy to popularize and popularizing, and improving adsorption performance.

Active Publication Date: 2021-04-13
SHENZHEN SINO ASSESSMENT GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual implementation process, the pretreatment method provided by the national standard needs to use a large number of highly pure organic reagents on the one hand, and these reagents will inevitably cause health hazards to experimenters; on the other hand, it also needs to use expensive Various solid-phase adsorption cartridges, these reagents and cartridges must be properly treated after use; in particular, the entire pretreatment process has many steps, takes a long time, is generally time-consuming and laborious, and results in a relatively low recovery rate And there are disadvantages such as high cost
There are patented technologies that are optimized in sample pretreatment, but in the detection process, high-end ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometers and other high-end instruments that are expensive, maintenance and maintenance, and use costs must be used Instruments, this technology is difficult to gain rapid popularization in a short period of time

Method used

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  • Accurate and rapid detection method for pesticide residues in fruits
  • Accurate and rapid detection method for pesticide residues in fruits
  • Accurate and rapid detection method for pesticide residues in fruits

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] A method for accurately detecting pesticide residues in fruits, comprising the following steps:

[0070] (1) Homogenize 20 g of apples with a homogenizer at 9500 rpm for 1 minute to obtain a slurry; take 5 g of the slurry in a 50 mL centrifuge tube with a stopper, then add 20 mL of deionized water and 2 mL of acetonitrile, and shake at 1800 rpm 2 minutes;

[0071] (2) Add 50 mg of magnetic nanomaterials to the stoppered centrifuge tube of step (1), and continue shaking at 1800 rpm for 4 minutes;

[0072] (3) The magnet is placed on the side wall of the centrifuge tube, the magnetic nanomaterials fully absorb the pesticides in the fruit, and the magnetic nanomaterials with magnetism are adsorbed on the side wall close to the magnet, and the mixed solution of the fruit, acetonitrile and water is discarded, Wash the magnetic nanomaterials 3 times with 2 mL deionized water each time, and discard the cleaning solution;

[0073] (4) Use 4 mL of organic solvent to ultrasonic...

Embodiment 2

[0085] A method for accurately detecting pesticide residues in fruits, comprising the following steps:

[0086] (1) Homogenize 20 g of apples with a homogenizer at 9500 rpm for 1 minute to obtain a slurry; take 5 g of the slurry in a 50 mL centrifuge tube with a stopper, then add 20 mL of deionized water and 2 mL of acetonitrile, and shake at 1800 rpm 2 minutes;

[0087] (2) Add 50 mg of magnetic nanomaterials to the stoppered centrifuge tube of step (1), and continue shaking at 1800 rpm for 4 minutes;

[0088] (3) The magnet is placed on the side wall of the centrifuge tube, the magnetic nanomaterials fully absorb the pesticides in the fruit, and the magnetic nanomaterials with magnetism are adsorbed on the side wall close to the magnet, and the mixed solution of the fruit, acetonitrile and water is discarded, Wash the magnetic nanomaterials 3 times with 2 mL deionized water each time, and discard the cleaning solution;

[0089] (4) Use 4 mL of organic solvent to ultrasonic...

Embodiment 3

[0106] A method for accurately detecting pesticide residues in fruits, comprising the following steps:

[0107] (1) Homogenize 20 g of apples with a homogenizer at 9500 rpm for 1 minute to obtain a slurry; take 5 g of the slurry in a 50 mL centrifuge tube with a stopper, then add 20 mL of deionized water and 2 mL of acetonitrile, and shake at 1800 rpm 2 minutes;

[0108] (2) Add 50 mg of magnetic nanomaterials to the stoppered centrifuge tube of step (1), and continue shaking at 1800 rpm for 4 minutes;

[0109] (3) The magnet is placed on the side wall of the centrifuge tube, the magnetic nanomaterials fully absorb the pesticides in the fruit, and the magnetic nanomaterials with magnetism are adsorbed on the side wall close to the magnet, and the mixed solution of the fruit, acetonitrile and water is discarded, Wash the magnetic nanomaterials 3 times with 2 mL deionized water each time, and discard the cleaning solution;

[0110] (4) Use 4 mL of organic solvent to ultrasonic...

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Abstract

The invention discloses an accurate and rapid detection method for pesticide residues in fruits. The accurate and rapid detection method comprises the following steps of: (1) carrying out pulp refining on fruits by using a refiner, and adding water and acetonitrile into the pulp for dispersion; (2) adding a magnetic nano-material into the dispersed pulp and carrying out continuous oscillation extraction; (3) separating the magnetic nano-material for extracting and adsorbing the pesticide from the fruit solution by using a magnet; (4) carrying out ultrasonic elution on the pesticide adsorbed by the magnetic nano material by adopting a small amount of organic reagent, and adding anhydrous sodium sulfate into eluent for dehydration; (5) adding an internal standard compound solution; and (6) analyzing and detecting a to-be-detected liquid by using a gas chromatograph-mass spectrometer. The accurate and rapid detection method for pesticide residues in the fruits has the advantages of being short in operation time, low in organic reagent consumption, low in analysis cost, accurate in analysis result and easy to popularize.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to an accurate and rapid detection method for pesticide residues in fruits. Background technique [0002] With the rapid growth of my country's economy and the improvement of people's living standards, the production and consumption of fruits have increased rapidly year by year. In order to meet product output and extend the quality assurance period, fruit farmers use pesticides and other preservatives in the production process to maximize economic benefits. With my country's restrictions on the use of organochlorine pesticides such as DDT and HCH, as well as the highly toxic methamidophos, the more common pesticides used by fruit farmers are mainly other organophosphates with relatively weak toxicity and pyrethroids with medium and low toxicity. Pesticides. The output of organophosphorus pesticides in my country accounts for one-third of the world's total, and the output of orga...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/14B82Y30/00
CPCG01N30/02G01N30/06G01N30/14B82Y30/00G01N2030/062G01N2030/045Y02A50/30
Inventor 宋国新
Owner SHENZHEN SINO ASSESSMENT GRP
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