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A kind of Cyclocarya paliurus extract and its application in the preparation of anti-coronavirus medicine

A technology of coronavirus and extract, applied in the field of Cyclocarya paliurus extract and its preparation of anti-coronavirus drugs, to achieve the effect of inhibiting cell pathology, low cost, and simple preparation method

Active Publication Date: 2022-04-26
WEST CHINA HOSPITAL SICHUAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, also do not see the report that Cyclocarya paliurus extract is used to prepare the medicine of anti-SARS-CoV-2 virus

Method used

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  • A kind of Cyclocarya paliurus extract and its application in the preparation of anti-coronavirus medicine
  • A kind of Cyclocarya paliurus extract and its application in the preparation of anti-coronavirus medicine

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1: the preparation of Cyclocarya paliurus leaf extract of the present invention

[0030] (1) Weigh 100 kg of Cyclocarya paliurus leaves dried in the shade at room temperature, add 80% ethanol with a solid-to-liquid ratio of 1:5 (w / w) after crushing, soak at room temperature for 4 hours, centrifuge at 4800 r / min for 10 minutes, and remove the supernatant. This step was repeated 2 times.

[0031] (2) Add water with a solid-liquid ratio of 1:4 (w / w) to the remaining precipitate after centrifugation to remove the supernatant, soak at 85°C for 4h, centrifuge at 4800r / min for 10min, and take the supernatant. This step was repeated 2 times.

[0032] (3) After concentrating the supernatant obtained in step (2), vacuum-dry at 50° C. for 24 hours to obtain 12.2 kg of powdery solid (named as extract 1).

[0033] Using ultraviolet-visible spectrophotometry ("Chinese Pharmacopoeia" 2015 edition general rule 0401), with rutin as a contrast, the content of flavonoids in t...

experiment example 1

[0039] Experimental example 1: Neutralization test of Cyclocarya paliurus leaf extract on 2019 novel coronavirus (SARS-CoV-2)

[0040] 1. Virus strains and drugs

[0041] Test virus strain: SARS-CoV-2 virus (BetaCoV / JS02 / human / 2020, virus strain isolated from clinical cases of new coronary pneumonia in Jiangsu Province).

[0042] Virus cultivation: The SARS-CoV-2 virus was isolated by culturing in the VeroE6 cell line. VeroE6 cells were seeded in 24-well cell culture plates in Dulbecco minimal essential medium (DMEM) medium containing 10% fetal bovine serum and antibiotics at 37°C, 5% CO 2 Incubate for 3-4 days until 80% of the well bottom is covered with a monolayer of cells. Inoculate 50ul of the virus stock solution into a 24-well plate and continue culturing for 3 days until the cells become lesions. The adherent monolayer VeroE6 cells were digested with 0.5% papain, and after ultrasonic disruption, they were centrifuged to remove the precipitate, and the supernatant wa...

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Abstract

The invention discloses an extract of Cyclocarya paliurus and its use in preparing anti-coronavirus medicines. In the Cyclocarya paliurus extract, the content of flavonoids is 20wt.%-40wt.%, the content of polysaccharides is 5wt.%-20wt.%, and the content ratio of flavones and polysaccharides is ≥1.5:1. Experimental results show that the Cyclocarya paliurus extract with specific flavonoid and polysaccharide content of the present invention has SARS-CoV-2 virus neutralizing activity, can effectively inhibit the cytopathy caused by SARS-CoV-2 virus, and protect cells from being infected by SARS-CoV-2. 2 virus infection. The Cyclocarya paliurus extract provided by the invention has broad application prospects in the preparation of anti-coronavirus (especially SARS-CoV-2 virus) drugs with good safety and low preparation cost.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to an extract of Cyclocarya paliurus and its use in preparing anti-coronavirus drugs. Background technique [0002] There are currently 7 types of coronaviruses that can infect humans: HcoV-229E, HcoV-NL63, HcoV-HKU1, HcoV-OC43, Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East Respiratory Syndrome Coronavirus ( MERS-CoV), 2019 novel coronavirus (2019-nCoV, SARS-CoV-2). Among them, SARS-CoV-2 is a new type of β-coronavirus, which belongs to single-stranded RNA virus, which is highly infectious and generally susceptible to the crowd. Although there is a certain understanding of the epidemiology, clinical characteristics, diagnosis, treatment, and prognosis of the new coronavirus pneumonia caused by SARS-CoV-2, the treatment still cannot meet the clinical needs, and there is a lack of specific drugs. Still pretty grim. [0003] Most of the existing antiviral drugs are...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/52A61P31/14A61K127/00
Inventor 刘杰孙美灵牟军黄春旭杨琪廖文廖敏钧曹婷顾芯瑕
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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