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Application of gene composition in preparation of gastric cancer diagnosis kit

A diagnostic kit and kit technology, applied in the field of disease diagnosis, can solve problems such as discomfort of patients

Inactive Publication Date: 2021-04-20
NANJING FANYIDA BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both of these methods will cause discomfort to the patient

Method used

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  • Application of gene composition in preparation of gastric cancer diagnosis kit
  • Application of gene composition in preparation of gastric cancer diagnosis kit
  • Application of gene composition in preparation of gastric cancer diagnosis kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015]First, test materials

[0016]The total RNA extraction kit was purchased from US Invitrogen.

[0017]Anti-transcript kits purchased from thermo fisher scientific.

[0018]RT-PCR measuring instrument is purchased from Germany Qiagen.

[0019]The primers were design synthesis by Beijing Bo Osen.

[0020]Second, the test method

[0021]1, general information

[0022]186 cases of gastric cancer samples, an average age (58.4 ± 9.2), male 92 cases, 94 female women; non-gastric cancer samples, an average age (56.9 ± 10.6), male 110, female 115 Among them, non-gastric cancer samples include 70 gastritis samples, 80 cases of gastric ulcers and 75 cases of health samples. Gender, no statistical differences.

[0023]Intogenous Standards: 1 The disease patients have confirmed by gastroscopy and / or pathological examination, healthy people are health checkups; 2 have no technical treatment, chemotherapy and other means for treatment; 3 age ≥ 18 years old. Excluding criteria: 1 Merge of malignant tumors in other ...

Embodiment 2

[0053]A kit for diagnosing gastric cancer comprising a reagent for determining serum lncRNA KHSRPP1 and LNCRNA Linc02384 content.

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Abstract

The invention discloses application of a gene composition in preparation of a gastric cancer diagnostic kit. It is found that the accuracy rate of serum lncRNA KHSRPP1 and lncRNA LINC02384 combined diagnosis for distinguishing gastric cancer and non-gastric cancer is 90% or above, and efficiency of the serum lncRNA KHSRPP1 and lncRNA LINC02384 combined diagnosis is remarkably superior to that of the serum lncRNA KHSRPP1 or lncRNA LINC02384 single diagnosis. Therefore, the serum lncRNA KHSRPP1 and lncRNA LINC02384 have an application prospect of being developed into the kit for diagnosing gastric cancer.

Description

Technical field[0001]The invention belongs to the field of disease diagnosis, and is specifically involved in the use of genotyles in the preparation of gastric cancer diagnostic kits.Background technique[0002]Gastric cancer is a common digestive malignant tumor in clinical, often confused with gastritis or gastric ulcer, most gastric cancer patients are in the middle and late stage, seriously affecting the therapeutic effect and prognosis. Therefore, effectively screening, it is necessary to find that gastric cancer has its necessity. At present, gastroscopy and gastric pathological examination are recognized in gastric cancer diagnosis technology. But both methods can cause discomfort in patients.[0003]The detection of peripheral hematological tumor markers has no trauma, repeatable, and can be used at the same time point, and is an ideal means for tumor diagnosis and follow-up.[0004]Long-chain non-coded RNA (lncRNA) is a non-encoded RNA having a length greater than 200 nucleotide...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
Inventor 朱阳晨陈均全
Owner NANJING FANYIDA BIOTECHNOLOGY CO LTD