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Liquid chip detection model construction method and device, analysis method and device

A technology for detecting models and liquid chips, applied in measuring devices, analytical materials, material excitation analysis, etc., can solve problems such as signal offset, air bubbles, fluid fluctuations, etc., and achieve the effects of reducing result errors, increasing fault tolerance, and improving accuracy

Active Publication Date: 2021-06-01
UB BIOTECHNOLOGY ZHEJIANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1. Polystyrene microsphere is a common polymer microsphere material, because of its small particle size, large specific surface area, strong adsorption, easy modification and modification, etc., it can be applied to flow fluorescence method, but because Polystyrene microspheres have strong adsorption, and the microspheres are very easy to stick together, resulting in the formation of large particle clusters, and at the same time reducing the content of target substances marked on the microspheres
[0006] 2. During the process of labeling fluorescence with polystyrene microspheres, the absolute uniformity of the fluorescence content cannot be guaranteed. Generally, the coefficient of variation of the expression peak of the microspheres is specified within 5% in the industry to measure the uniformity of the microspheres, but In the microspheres stored for a long time, due to the uniformity problem, there will still be uneven fluorescent content of the microspheres, resulting in abnormal detection results of the target substance
Both the fluid system and the optical system in the system have equipment stability problems, especially the fluid system, which will cause fluid fluctuations, air bubbles, signal offset and other problems due to problems such as use time, pipeline pollution degree and detection sample concentration, etc. The situation will affect the signal stability of polystyrene microspheres, which will eventually lead to the error of the results

Method used

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  • Liquid chip detection model construction method and device, analysis method and device
  • Liquid chip detection model construction method and device, analysis method and device
  • Liquid chip detection model construction method and device, analysis method and device

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Experimental program
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Effect test

Embodiment 1

[0051] This embodiment provides a method for building a liquid chip detection model:

[0052] 1. Obtain raw data of a plurality of standard samples with different concentrations; wherein, each of the raw data includes: a plurality of continuous time series, and a fluorescence intensity value corresponding to each of the time series.

[0053] see figure 1 and figure 2 , figure 1 is the FSC-SSC plot of the raw data, used to circle the microspheres, figure 2 The microspheres are distinguished by the fluorescence signal of APC, and the fluorescence intensity value is displayed by PE.

[0054] 2. The fluorescence intensity value of the first time series point is retained as valid data, starting from the fluorescence intensity value of the second time series point, the fluorescence intensity value corresponding to each time series of each standard sample is judged Whether the signal condition is met; when the fluorescence intensity value satisfies the signal condition, the flu...

Embodiment 2

[0062] This embodiment provides an analysis method for a liquid chip:

[0063] 1. Obtain the fluorescence intensity value of the sample to be tested;

[0064] 2. Obtain the concentration value of the sample to be detected according to the standard curve provided by the fluorescence intensity value and embodiment 1.

[0065] According to the above method, 4 samples to be tested were detected, and the obtained data are shown in Table 1.

[0066] Table 1 Test results of 4 samples to be tested

[0067]

Embodiment 3

[0069] This embodiment provides a device for constructing a liquid chip detection model, including:

[0070] An acquisition module, configured to acquire raw data of a plurality of standard samples of different concentrations; wherein, each of the raw data includes: a plurality of continuous time series, and a fluorescence intensity value corresponding to each of the time series;

[0071] A denoising module, configured to perform denoising according to the plurality of time series of each of the standard samples and the corresponding plurality of fluorescence intensity values, to obtain a correction value of the fluorescence intensity of each of the standard samples;

[0072] The construction module is used to construct and obtain the detection model according to the calibration value of the fluorescence intensity of each standard sample and the standard concentration value of the standard sample.

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Abstract

The invention discloses a liquid chip detection model construction method and device, an analysis method and a device, comprising: obtaining raw data of a plurality of standard samples with different concentrations; wherein, each of the raw data includes: a plurality of continuous time series, and the fluorescence intensity values ​​corresponding to each of the time series; according to the multiple time series of each of the standard samples and the corresponding multiple fluorescence intensity values, denoising is performed to obtain each of the standard The fluorescence intensity correction value of the sample; the detection model is obtained by constructing the fluorescence intensity correction value of each standard sample and the standard concentration value of the standard sample. The present invention proposes a method for constructing a time series signal model, which can distinguish the data with stable particle size signals and perform subsequent analysis, and reduce the result error caused by the adhesion of microspheres.

Description

technical field [0001] The invention relates to the technical field of biology and new medicine, in particular to a liquid chip detection model construction method and device, analysis method and device. Background technique [0002] Liquid chip technology has also become suspended lattice technology, mainly led by the liquid chip developed by Luminex Company of the United States. It combines coded microspheres, laser technology, applied fluidics, the latest high-speed digital signal processor and computer algorithms, and has With the advantages of high throughput, high speed, low cost, high sensitivity, high precision, wide linear range, and easy operation, it is widely used in immunoassay, nucleic acid analysis, enzymatic analysis, receptor and ligand recognition and other analysis. [0003] The realization principle of the liquid chip (LuminexxMap) technology is to encode polystyrene microspheres with fluorescent dyes, and adjust the ratio of two different fluorescent dye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N15/00G01N15/10G01N15/14
Inventor 李国平严崇虎刘正权
Owner UB BIOTECHNOLOGY ZHEJIANG CO LTD