Application of beraprost sodium as medicine for improving vascular dementia
A kind of beraprost sodium, vascular dementia technology, applied in the application field of medicine
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[0033] Preparation of mouse vascular dementia model
[0034] Step 1: Anesthetize male B6 mice aged 8-9 weeks, about 20-25g, anesthetize the mice at 0.003ml / g chloral hydrate.
[0035] Step 2: Separation After anesthesia, the mice were fixed supine on the operating table, sheared and disinfected, and the right common carotid artery was separated by using a median incision in the neck.
[0036] Step 3: Ligation Through a median incision in the neck, the right common carotid artery is exposed and picked out of the tissue with microscopic curved forceps. Place a 7-0 silk suture around the distal end of the right common carotid artery. (Tie a slipknot, untie it after placing the spring coil and take it out).
[0037]Step 4: Coil Placement The artery is gently lifted by gripping the suture with curved forceps and the common carotid artery is placed between the coils, which are wound by rotating around the common carotid artery. The slipknot was then undone to see the common carot...
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