Test method for rapidly detecting escherichia coli

A technology of Escherichia coli and test method, which is applied in the field of Chinese patent medicine preparation, can solve the problems of affecting the accuracy of the test, affecting the safety of taking, and the variation of drug properties, so as to avoid the influence of impurities on the test, increase the reliability of culture, and have suitable living conditions for strains. Effect

Pending Publication Date: 2021-04-23
陕西唐王天洋制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Both Escherichia coli and coliform bacteria are bacteria that exist in the intestines and feces of warm-blooded animals. They are conditional pathogenic bacteria that mainly cause water pollution and may also cause food poisoning. The main intestinal pathogenic bacteria are similar, and its appearance may also indicate the existence of some intestinal pathogenic bacteria. Therefore, this bacterium is an internationally recognized indicator bacterium for health monitoring. In the preparation of Chinese patent medicines, polluted water is used for preparation. It is easy to cause the pollution of Chinese patent medicines and cause loss of medicine and variation of medicine properties, which will affect the safety of later administration
[0003] Existing Escherichia coli test method adopts culture observation and staining test method to carry out test detection to Escherichia coli, its sample is cultivated in the lactose fermentation tube in dyeing, observes bacterial colony, if gas is produced in the lactose fermentation tube, Gram staining is Negative non-bacillus, that is, Escherichia coli positive generally adopts aseptic operation. In the process of this test method, the sample needs to be treated correspondingly, diluted to a certain ratio, and then cultivated under certain conditions. According to the biochemical indicators such as the color and shape of the colony, the number of colonies on the plate is calculated. In the process of culturing the sample, the test method only puts the sample under sterile conditions, and through dilution and medium Cultivate and observe a certain number of colonies at the place where they are produced. Since the acid-base adjustment of the sample plate is not carried out by sodium hydroxide and hydrogen chloride, the acid-base of the sample cannot be stabilized in a certain range when the pH value of the sample homogeneous solution is affected. To solve the problem of the accuracy of the test, we propose a rapid test method for the detection of Escherichia coli

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The invention provides a technical scheme: a test method for rapid detection of Escherichia coli, the method is as follows:

[0024] Step 1: Dissolve the sample with physiological saline under sterile conditions, and rinse the dissolved sample solution repeatedly with physiological saline;

[0025] Step 2: Dissolve and disperse the sample in a high-speed disperser after dissolving and washing with physiological saline;

[0026] Step 3: The centrifuged sample is then subjected to multi-membrane centrifugal filtration with a filter membrane, and the filtrate is obtained after filtration. The filtrate is divided into several groups of sterile test tubes, and distilled water is added to the sterile test tubes for dilution treatment;

[0027] Step 4: The pH value of the diluted sample solution is measured under sterile conditions to obtain the pH value of the diluted sample solution, and the acid-base adjustment treatment is performed according to the value of the pH value; ...

Embodiment 2

[0037] The invention provides a technical scheme: a test method for rapid detection of Escherichia coli, the method is as follows:

[0038] Step 1: Dissolve the sample with physiological saline under sterile conditions, and rinse the dissolved sample solution with physiological saline repeatedly;

[0039] Step 2: Put the sample dissolved and washed in physiological saline into a high-speed disperser for dispersion;

[0040] Step 3: The centrifuged sample is then subjected to multi-membrane centrifugal filtration with a filter membrane, and the filtrate is obtained after filtration. The filtrate is divided into several groups of sterile test tubes, and distilled water is added to the sterile test tubes for dilution treatment;

[0041] Step 4: The pH value of the diluted sample solution is measured under sterile conditions to obtain the pH value of the diluted sample solution, and the acid-base adjustment treatment is performed according to the value of the pH value;

[0042] S...

Embodiment 3

[0051] The invention provides a technical scheme: a test method for rapid detection of Escherichia coli, the method is as follows:

[0052] Step 1: Dissolve the sample with physiological saline under sterile conditions, and rinse the dissolved sample solution repeatedly with physiological saline;

[0053] Step 2: Dissolve and disperse the sample in a high-speed disperser after dissolving and washing with physiological saline;

[0054] Step 3: The centrifuged sample is then subjected to multi-membrane centrifugal filtration with a filter membrane, and the filtrate is obtained after filtration. The filtrate is divided into several groups of sterile test tubes, and distilled water is added to the sterile test tubes for dilution treatment;

[0055] Step 4: The pH value of the diluted sample solution is measured under sterile conditions to obtain the pH value of the diluted sample solution, and the acid-base adjustment treatment is performed according to the value of the pH value; ...

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Abstract

The invention discloses a test method for rapidly detecting escherichia coli. The test method comprises: step I, dissolving a sample with normal saline under an aseptic condition, and repeatedly washing the dissolved sample solution with the normal saline; step II, dispersing the sample dissolved and cleaned with the normal saline in a high-speed dispersion machine; and step III, performing multi-membrane centrifugal filtration on the centrifuged sample by using a filter membrane to obtain a filtrate, sub-packaging the filtrate into a plurality of groups of sterile test tubes, and adding distilled water into the sterile test tubes to perform dilution treatment. According to the invention, designed acid-base regulating agents are respectively 1mol / L NaOH and 1mol / L HCl, and the acidity of a sample solution is regulated, so that the survival condition of a bacterial strain in the sample is more suitable, the culture reliability of the bacterial strain is improved, and influence of inactivation of the bacterial strain on the experimental result is avoided.

Description

technical field [0001] The invention belongs to the technical field of Chinese patent medicine preparation, in particular to a test method for rapid detection of Escherichia coli. Background technique [0002] Both Escherichia coli and coliform bacteria exist in the intestines and feces of warm-blooded animals. They are opportunistic pathogens that mainly cause water pollution and may also cause food poisoning. The main intestinal pathogens are similar, and its appearance may also indicate the existence of some intestinal pathogens. Therefore, this bacteria is an internationally recognized indicator bacteria for health monitoring. In the preparation of Chinese patent medicines, the use of polluted water sources is extremely It is easy to cause contamination of proprietary Chinese medicines, resulting in loss of medicinal properties and variation in medicinal properties, which affects the safety of later use. [0003] The existing test method for Escherichia coli adopts cult...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12Q1/10C12R1/19
CPCC12Q1/10C12Q2304/40G01N2333/245
Inventor王天宁侯少华王显杰何彩强余志华
Owner陕西唐王天洋制药有限公司