Application of intestinal flora in nerve injury detection

A technology for nerve damage and central nervous system, applied in measuring devices, microbe-based methods, microbiological measurement/inspection, etc., can solve problems such as the relationship between intestinal flora that has not been disclosed in the literature, achieve high specificity, simple operation, The effect of strong sensitivity

Active Publication Date: 2021-04-27
温州医科大学慈溪生物医药研究院
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Problems solved by technology

[0006] At present, there is no literature disclosin...

Method used

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  • Application of intestinal flora in nerve injury detection

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Experimental program
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Effect test

Embodiment 2

[0037] Example 2 Fluorescence quantitative PCR verification related flora

[0038] 1. Perform large-scale QPCR verification on the above-mentioned flora, and collect 30 cases of feces samples from healthy people and patients with central nervous system damage according to the sample collection method in Example 1.

[0039] 2. Extraction and quantification of fecal bacterial DNA

[0040] Bacterial DNA was extracted from stool samples using a stool genomic DNA extraction kit, and the operation steps were carried out according to the instructions;

[0041] Genomic DNA was detected by 1% agarose gel electrophoresis to determine the total DNA concentration and purity of the extracted fecal bacteria, and the mass concentration of DNA in all samples was unified to 100 mg / L.

[0042] 3. Real-time fluorescence quantitative PCR

[0043] 1) Primer design and synthesis

[0044] According to the sequence of the V3-V4 region of the bacterial 16SrRNA gene, yes. Specific primers were design...

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Abstract

The invention relates to an application of intestinal flora in nerve injury detection. The intestinal flora is pseudomonas aeruginosa, veillonella and streptococcus digestive. According to the invention, the horizontal change of the specific flora in nerve injury is firstly discovered, the nerve injury diagnosis can be achieved by detecting the change of the specific flora, and with the application of the microbial marker to diagnose, the operation is simple, the specificity is high, and the sensitivity is strong.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to the application of intestinal flora in the detection of nerve damage. Background technique [0002] Central nervous system injury (including brain injury caused by cerebral apoplexy or cerebrovascular accident, traumatic brain injury and spinal cord injury) is a major disease that threatens human health. It results in severe disability and an unusually high mortality rate, as well as enormous medical rehabilitation costs. For the diagnosis of central nervous system injury, usually rely on X-ray head film, skull base tomography, and CT scan to infer cranial nerve injury through the direction of the fracture line; MRI skull base thin-layer scan can also occasionally show nerve root swelling, bleeding, and fracture Happening. Overall, this detection method has the disadvantages of difficult operation and high cost, and it usually requires professional pers...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/04C12N15/11C12R1/385C12R1/46C12R1/01
CPCC12Q1/689G01N2800/28
Inventor 张宏宇龚方华肖健黄鹏吴艳青谢玲
Owner 温州医科大学慈溪生物医药研究院
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