Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Buffer and preparation method for preservation of superparamagnetic particles and their protein conjugates

A buffer, superparamagnetic technology, applied in the field of protein preservation, can solve the problems of difficult magnetic particles, repeatability deviation, mixing, etc.

Active Publication Date: 2021-09-28
SOPHONIX CO LTD
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for reaction consumables with special shapes (such as V-shaped bottom reaction cups), this method is not easy to mix the magnetic particles, which will cause large repeatability deviations

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Buffer and preparation method for preservation of superparamagnetic particles and their protein conjugates
  • Buffer and preparation method for preservation of superparamagnetic particles and their protein conjugates
  • Buffer and preparation method for preservation of superparamagnetic particles and their protein conjugates

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0037] (1) Weigh 500ml of purified water, add 5.6g of disodium hydrogen phosphate dodecahydrate, 0.55g of sodium dihydrogen phosphate, 9g of sodium chloride, 30g of bovine serum albumin, 70g of sucrose and 3ml of Tween-20; stir to dissolve ; Make base buffer;

[0038] (2) Add 0.3g of xanthan gum, 0.5g of sodium alginate, and 15g of gelatin to the basic buffer in sequence, stir and heat at 35°C for 12h, adjust the pH value of the solution to 7.0, add purified water to 1L, and use 0.22μm Filter through a filter membrane to obtain the buffer solution.

Embodiment 2

[0040] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0041] (1) Weigh 500ml of purified water, add 5.9g of disodium hydrogen phosphate dodecahydrate, 0.60g of sodium dihydrogen phosphate, 5g of sodium chloride, 10g of bovine serum albumin, 135g of sucrose and 1ml of Tween-60; stir to dissolve ; Make base buffer;

[0042] (2) Add 0.5g of xanthan gum, 0.5g of sodium alginate, and 5g of gelatin to the basic buffer in turn, stir and heat at 42°C for 16h, adjust the pH value of the solution to 7.6, add purified water to 1L, and use 0.2μm Filter through a filter membrane to obtain the buffer solution.

Embodiment 3

[0044] A buffer for the preservation of superparamagnetic microparticles and their protein connectors, comprising the following steps:

[0045] (1) Take 500ml of purified water, add 4g of disodium hydrogen phosphate dodecahydrate, 1g of sodium dihydrogen phosphate, 5g of sodium chloride, 1g of bovine serum albumin, 150g of sucrose and 1ml of Tween-80; stir to dissolve; into a basic buffer;

[0046] (2) Add 0.1g of xanthan gum, 0.1g of sodium alginate, and 1g of gelatin to the basic buffer in turn, stir and heat at 30°C for 16h, adjust the pH value of the solution to 7.2, add purified water to 1L, and use 0.5μm Filter through a filter membrane to obtain the buffer solution.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of protein preservation, and in particular relates to a buffer and a preparation method for the preservation of superparamagnetic particles and their protein junctions. The buffer used for the preservation of superparamagnetic particles and their protein connectors includes the following components: disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate, sodium chloride, bovine serum albumin, sucrose, xanthan gum , sodium alginate, gelatin. The buffer provided by the present invention can store the connection between micron-sized superparamagnetic particles and superparamagnetic particles and specific proteins for a long time, so that it will not fail due to physical effects (such as sedimentation and hardening); it is a kind of Special buffer for long-term storage of superparamagnetic particles and their protein conjugates.

Description

technical field [0001] The invention belongs to the technical field of protein preservation, and in particular relates to a buffer and a preparation method for the preservation of superparamagnetic particles and their protein junctions. Background technique [0002] Magnetic particle chemiluminescence is an advanced immunological detection method that can detect specific protein components in blood in vitro. This method can effectively assist doctors in assisting diagnosis of patients' conditions. Protein conjugation to micron-sized superparamagnetic particles plays an important role in this methodology. [0003] However, the protein conjugates of superparamagnetic particles are very easy to settle and because of their poor hydrophilicity, they will gradually compact into a sheet or block structure after settling. The hardened magnetic particle-protein linker is very unstable, which will cause the overall failure of the reaction system and lead to product failure. At pres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531G01N33/543
CPCG01N33/531G01N33/54326
Inventor 王法龙孙家刘聪李博飞
Owner SOPHONIX CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com