Sialic acid nanoparticle as well as preparation method and application thereof
A nanoparticle and sialic acid technology, which is applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of reducing clearance, changing viscosity, and poor patient dependence
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Embodiment 1
[0038] The preparation of embodiment 1 sialic acid nanoparticles
[0039] Take 750 mg of polystyrene maleic anhydride copolymer (PMSA) with a molecular weight of 10000 and dissolve it in 10 mL of DMF, then add 350 mg of aminosialic acid (SA, x=2), and add 0.8 mL of triethylamine to the above In the reaction solution, stir the reaction at room temperature for 12 hours, then add 8 mL of saturated NaHCO 3 Aqueous solution, the reaction solution can be collected after stirring at room temperature for 1 hour; when collecting the reaction solution, use a dialysis bag with a molecular weight cut-off of 10,000 for dialysis for 24 hours, the dialysate is ultrapure water, and the medium is changed 4-5 times. Finally, the solid substance obtained by freeze-drying the solution in the dialysis bag is the sialic acid polymer compound.
[0040] 10 mg of the above-mentioned sialic acid polymer compound was dissolved in 10 ml of ultrapure water, and ultrasonic (40 KHz, 25° C.) was used for 1 ...
Embodiment 2
[0042] The preparation of embodiment 2 sialic acid nanoparticles
[0043]Take 1000 mg of polystyrene-maleic anhydride copolymer (PMSA) with a molecular weight of 5000 and dissolve it in 10 mL of DMF, then add 500 mg of aminosialic acid (SA, x=4), and add 0.5 mL of triethylamine to the above In the reaction solution, stir the reaction at room temperature for 12 hours, then add 10 mL of saturated NaHCO 3 Aqueous solution, the reaction solution can be collected after stirring at room temperature for 1 hour; when collecting the reaction solution, use a dialysis bag with a molecular weight cut-off of 5000 for dialysis for 24 hours, the dialysate is ultrapure water, and the medium is changed 4-5 times. Finally, the solid substance obtained by freeze-drying the solution in the dialysis bag is the sialic acid polymer compound.
[0044] 10 mg of the above-mentioned sialic acid polymer compound was dissolved in 10 ml of ultrapure water, and ultrasonic (40 KHz, 25° C.) was used for 1 ho...
Embodiment 3
[0046] The preparation of embodiment 3 sialic acid nanoparticles
[0047] Take 500 mg of polystyrene-maleic anhydride copolymer (PMSA) with a molecular weight of 20,000 and dissolve it in 10 mL of DMF, then add 200 mg of aminosialic acid (SA, x=4), and add 1 mL of triethylamine to the above reaction solution, stirred at room temperature for 12 hours, then added 10 mL of saturated NaHCO 3 Aqueous solution, the reaction solution can be collected after stirring at room temperature for 1 hour; when collecting the reaction solution, use a dialysis bag with a molecular weight cut-off of 20,000 for dialysis for 24 hours, the dialysate is ultrapure water, and the medium is changed 4-5 times. Finally, the solid substance obtained by freeze-drying the solution in the dialysis bag is the sialic acid polymer compound.
[0048] 10 mg of the above-mentioned sialic acid polymer compound was dissolved in 10 ml of ultrapure water, and ultrasonic (40 KHz, 25° C.) was used for 1 hour to obtain ...
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