Unlock instant, AI-driven research and patent intelligence for your innovation.
A kind of phospholipid microbead as artificial antigen presenting cell and its preparation method and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of artificial antigens and microbeads, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve problems such as application limitations, difficulty in large-scale acquisition of DCs, and difficulty in controlling the differentiation state, to achieve increased interaction, Strong adsorption capacity, the effect of improving the amount and quality of T cell expansion
Active Publication Date: 2022-05-17
CARBIOGENE THERAPEUTICS CO LTD
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, it is difficult to control the differentiation state of DCs under in vitro culture conditions, and it is difficult to obtain DCs from patients' autologous sources on a large scale
In the context of the general trend of research and development of allogeneic universal cell therapy products, the application of DC is greatly limited
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
preparation example Construction
[0032] A method for preparing phospholipid microbeads as artificial antigen-presenting cells, comprising the steps of:
[0033] Prepare materials according to recipes;
[0034] Dissolving the agar in hot water, as a preferred method, the hot water is hot water at 60°C to form an agar solution, and then add gelatin to dissolve it;
[0035] Adding phosphatidylcholine and polyethylene glycol monomethyl ether to ethanol and stirring and mixing, as a preference, the rotation speed is 200 rpm to obtain the first mixed solution;
[0036] Mix the agar-gelatin mixed solution with an equal volume of organic solvent, stir, and as a preferred method, the rotating speed is 380 rpm to obtain the second mixed solution; as an example, the organic solvent includes: butyl acetate, chloroform, gasoline ; As a preference, it is better to use ethyl acetate as non-toxic.
[0037] Pour the first mixed solution into the second mixed solution, and stir, as a preferred method, at a speed of 380 rpm t...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
PUM
Property
Measurement
Unit
particle diameter
aaaaa
aaaaa
specific surface area
aaaaa
aaaaa
Login to View More
Abstract
The invention discloses a phospholipid microbead used as an artificial antigen-presenting cell and its preparation method and application. The formula of the phospholipid microbead includes: 32-36 parts of agar, 20-24 parts of gelatin, 26-30 parts Phosphatidylcholine, 28-32 parts of magnetic powder, 6-10 parts of polyethylene glycol monomethyl ether; preparation method, including: configuring agar-gelatin mixed solution; adding phosphatidylcholine and polyethylene glycol monomethyl ether in ethanol The ether surfactant is stirred and mixed; the agar-gelatin mixed solution is added to the organic solvent and then mixed with the surfactant, then the magnetic powder is added, stirred, and ice water is added to obtain the phospholipid microbeads; the phospholipid microbeads of the present invention have uniform adsorption force to nucleic acid , and has a strong adsorption capacity, which increases the contact opportunities between nucleic acids and nucleic acids, and between nucleic acids and cytokines, and can be applied to the expansion of T cells based on artificial antigen-presenting cells, which can increase the amount and quality of amplification.
Description
technical field [0001] The invention relates to the field of biological materials, in particular to a phospholipid microbead used as an artificial antigen-presenting cell, a preparation method thereof and an application thereof. Background technique [0002] In a normal immune response, antigen-specific T cells require stimulation by at least two signals to proliferate and mount an immune response to the antigen. The first signal comes from the recognition of MHC / antigen peptide complex by TCR, which transmits the antigen-specific recognition signal; the second signal is provided by costimulatory molecules of antigen-presenting cells (APC), which is a non-specific co-stimulatory signal. In the process of recognizing and binding with APC, a variety of transmembrane molecules gather on the "raft" structure rich in sphingomyelin and cholesterol, and approach each other to form clusters, forming the site where cells bind to each other. synapse. The formation of immune synapse ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More