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Method for determining concentration of omeprazole in plasma by liquid chromatography-mass spectrometry

A technology of omeprazole and LC/MS, applied in the field of medicine, can solve the problems of speed, precision, sensitivity, and selectivity need to be improved, and achieve the effects of high sensitivity, fast method and easy operation.

Inactive Publication Date: 2021-05-11
徐州立顺康达医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the speed, precision, sensitivity and selectivity of the existing determination methods for omeprazole need to be improved

Method used

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  • Method for determining concentration of omeprazole in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of omeprazole in plasma by liquid chromatography-mass spectrometry
  • Method for determining concentration of omeprazole in plasma by liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Example: Human K 2 Determination of Omeprazole Concentration in EDTA Plasma

[0032] 1. Experimental materials and analytical equipment

[0033] Omeprazole (analyte): China National Institutes for Food and Drug Control or the same or higher grade standard Omeprazole-d3 (internal standard): TLC Pharmaceutical Standards or the same or higher grade standard Reagents used see Table 1 below:

[0034] Table 1 Reagent Details

[0035] Reagent name level manufacturer Acetonitrile (ACN) HPLC J. T. Baker Methanol (MeOH) HPLC J. T. Baker Ammonium acetate (CH 3 COONH 4 )

HPLC J. T. Baker Ammonium Hydroxide (NH 4 Oh a )

ACS J. T. Baker

[0036] a: NH 4 OH is expressed as A.C.S. grade ammonia water, and its ammonia content is 28-30%.

[0037] Note: Reagents of the same grade or higher can also be used

[0038] The analytical equipment used is shown in Table 2 below:

[0039] Table 2 Details of equipment used

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Abstract

The invention discloses a method for determining the concentration of omeprazole in plasma by liquid chromatography-mass spectrometry. The method adopts a liquid chromatography-mass spectrometry system for determination and comprises the steps of firstly taking a sample to be detected, adding a certain amount of mixed organic solvent for extraction and pretreatment, separating by a chromatographic column, and detecting by a mass spectrometry detector. The method disclosed by the invention is rapid, accurate, high in sensitivity and simple and convenient to operate, and provides a basis for measuring the blood concentration of omeprazole; the linear range of a plasma standard curve of the method is 2 to 1500ng / mL, the intra-batch and inter-batch precision RSD is less than + / -15%, and the method is suitable for determining the concentration of omeprazole in plasma.

Description

technical field [0001] The invention belongs to the technical field of medicines, in particular to a method for determining the concentration of omeprazole in blood plasma by liquid mass spectrometry. Background technique [0002] Omeprazole is a substituted benzimidazole compound, which is a racemate of a pair of active optical enantiomers. Omeprazole reduces gastric acid secretion by acting on the proton pump in parietal cells through a special mechanism, and this effect is reversible. Omeprazole is a weak base that is concentrated and converted into an active form in the acidic environment of the parietal cells, inhibiting the final link in the production of hydrochloric acid in gastric juice: H+-K+-ATPase, the inhibitory effect is dose-dependent, and has a dose-dependent effect on Both basal and stimulated gastric acid secretion had a role, independent of the type of stimulus. Omeprazole has no effect on cholinergic and histamine receptors. Similar to H2 receptor bloc...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 范志鸿
Owner 徐州立顺康达医药科技有限公司
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