Russula virescens symbiotic fungus M2-1 as well as fungicide and fermentation liquor extract thereof

A technology of symbiotic fungi and cyanobacteria, applied in the field of microorganisms, can solve the problems of lack of antibacterial activity cyanobacteria symbiotic fungi and the like

Active Publication Date: 2021-05-18
YUNNAN ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is a lack of a symbiotic fungus M

Method used

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  • Russula virescens symbiotic fungus M2-1 as well as fungicide and fermentation liquor extract thereof
  • Russula virescens symbiotic fungus M2-1 as well as fungicide and fermentation liquor extract thereof
  • Russula virescens symbiotic fungus M2-1 as well as fungicide and fermentation liquor extract thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as Figure 1 to Figure 9 As shown, the present invention screens and obtains a kind of symbiotic fungus M2-1 (Mucorfragilis brittle mold) of P. of. The fermentation liquid extract of its strain culture is pathogenic to Bacillus lentus, Vibrio parahaemolyticus, Staphylococcus hemolyticus, Pseudomonas aeruginosa, Shigella flexneri, Bacillus subtilis, Escherichia coli, etc. Bacteria have good antibacterial activity. In order to alleviate the threat of pathogenic bacteria drug resistance to human health, research and development of new antibacterial drugs provide a new way.

[0043] Isolation and identification of the symbiont M2-1 strain of Pleurotus chinensis

[0044] 1. Isolation of Symbiotic Bacteria of Pseudomonas

[0045] Rinse the fruiting body of Pleurotus chinensis with tap water earlier for 36 hours, then transfer to the aseptic workbench, and then rinse the fruiting body with 3L sterile water.

[0046] Use a sterilized knife to take 2g of Pleurotus spor...

Embodiment 2

[0058] Extraction of Active Substances from Symbiotic Bacteria M2-1

[0059] 1. Liquid fermentation culture

[0060] Liquid fermentation medium LMM: lactose 15g / L, sodium nitrate 6g / L, potassium chloride 0.52g / L, magnesium sulfate heptahydrate 0.52g / L, potassium dihydrogen phosphate 1.52g / L, trace elements 1ml / L, pure Water 1L. Media aliquots.

[0061] Add 700lul of MY liquid to a 2ml centrifuge tube, then scrape an appropriate amount of mycelium of Pleurotus chinensis M2-1 into the centrifuge tube, crush it for 90 seconds, and then inoculate the crushed solution into each tube Place in a Erlenmeyer flask with LMM liquid medium in a constant temperature shaking incubator at 150r min-1, and culture at 28°C for 10 days

[0062] 2. Extraction of cultured mycelium

[0063] After the fermentation culture is completed, the obtained fungal mycelium and culture solution. Use a separatory funnel to separate the mycelium and the bacterial liquid, add ethyl acetate (1:1) to the bact...

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Abstract

The invention discloses a russula virescens symbiotic fungus M2-1 as well as a fermentation liquor extract of a strain culture thereof. According to a strain of russula virescens symbiotic fungus M2-1, the strain is the russula virescens symbiotic fungus M2-1 (Mucor fragilia). The preservation name is mucor fragile YAFM2-1; the strain is preserved in the China Center for Type Culture Collection, and the preservation address is Wuhan University, Wuhan, China; the preservation date is July 16, 2020; and the preservation number is CCTCC NO: M 2020322. The symbiotic fungus has a relatively good inhibiting effect on various kinds of pathogenic bacteria and provides a new way for relieving the threat of drug resistance of pathogenic bacteria to human health and researching and developing new antibacterial drugs.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a symbiotic fungus M2-1 of Pleurotus chinensis and its bacterial agent and fermentation liquid extract. Background technique [0002] Pathogenic bacteria can make people, animals and plants sick, and bring us huge health, food and property losses every year. In order to kill germs or inhibit the activity of germs, people have invented antibiotics and synthetic antibacterial drugs. With the widespread use of antibacterial drugs application, pathogenic bacteria are resistant to antibiotics, and even multi-drug resistance has emerged. The occurrence and spread of pathogenic bacteria resistance has constituted a serious threat to human health, so looking for emerging biological resources is a promising path. At present, there are more and more studies on the secondary metabolites of symbiotic bacteria. During the long-term co-evolution process, symbiotic bacteria and host pla...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K36/06A61P31/04C12R1/785
CPCC12N1/14A61K36/06A61P31/04
Inventor 王毅罗婷
Owner YUNNAN ACAD OF FORESTRY
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