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A molecular marker for heat-resistant properties of cucurbits and its application

A technology of molecular markers and heat-resistant traits, applied in the field of crop stress tolerance identification, can solve the problems of unreliable accuracy of results, uneven treatment conditions and plant growth, and achieve high correlation results

Active Publication Date: 2022-07-05
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, judging the heat-resistant characteristics of gourds through existing methods requires heat treatment of plants, which may be affected by treatment conditions and uneven plant growth, and the accuracy of the results needs to be improved

Method used

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  • A molecular marker for heat-resistant properties of cucurbits and its application
  • A molecular marker for heat-resistant properties of cucurbits and its application
  • A molecular marker for heat-resistant properties of cucurbits and its application

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Embodiment 1

[0031] Phenotypic data statistics, F2 population was constructed by heat-resistant cucurbit L1 and heat-labile cucurbit L6. After the parent and colony were sown, the heat treatment was started when the plant grew to two leaves and one center. The heat treatment conditions are: 40° C., 80% relative humidity, light intensity of 30,000 lux, 6h.

[0032] The electrical conductivity (EC) of gourd leaves before and after heat treatment was determined. The leaves were first washed with deionized water, cut into sections, soaked in deionized water for 30 min, and the conductivity was measured by a conductivity meter (PHSJ-3F, Shanghai Precision Instruments), which was recorded as S1. After that, the blade samples were heated for 15 min, and the electrical conductivity was measured, which was recorded as S2. The relative conductivity (REC) was calculated by the formula (S1 / S2)×100%. The leaf injury rate (LRI) was calculated by the formula (Lt-Lck) / (100-Lck)×100%, where Lt and Lck r...

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Abstract

The invention discloses a molecular marker for heat-resistant properties of cucurbits and its application. It belongs to the technical field of crop stress tolerance identification. Including SNP1, SNP2 or SNP3. The invention can carry out molecular marker identification for heat-resistance characteristics of cucurbits at the seedling stage. The molecular markers related to the heat resistance characteristics of cucurbits obtained by resequencing combined with bioinformatics were highly correlated with the heat resistance characteristics of cucurbits.

Description

technical field [0001] The invention relates to the technical field of crop stress tolerance identification, and more particularly to a molecular marker for heat-resistant characteristics of cucurbits and its application. Background technique [0002] At present, the global climate is warming, and crop growth is facing severe high temperature challenges. Identification of heat-resistant characteristics of cucurbits is a key link in breeding heat-resistant cucurbit varieties. The commonly used method to identify the heat resistance characteristics of cucurbits is to treat the cucurbit plants at high temperature, and judge the heat resistance characteristics of the cucurbits according to the changes in the phenotype and biochemical indicators of the plants. [0003] However, judging the heat-resistant characteristics of cucurbits by the existing method requires heat treatment of the plants, which may be affected by treatment conditions and uneven plant growth, and the accurac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 宋慧古斌权张蕾琛黄芸萍
Owner NINGBO ACAD OF AGRI SCI