Application of sodium houttuyfonate in preparation of medicine for preventing and treating nervous system diseases taking Kv7/M channel as target spot
A neurological disease, a technology for sodium houttuyniacin, which is applied in the field of drugs for neurological diseases, and can solve the problems of undiscovered antiepileptic activity, analgesia, and changing neuronal excitability of houttuynia sodium or any derivative thereof. , to achieve the effects of good toxicological safety, small side effects and stable metabolism
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Embodiment 1
[0024] Electrophilic tester
[0025] (1) Test compound: sodium fish
[0026] (2) Test method: Culture of Chinese hamster ovary cells (CHO): CHO cells in KCNQ2 / Q3 channels are cultured in 10% fetal bovine serum, 500 ug / ml hygromycin and g418, 100 u / ml penicillin and chain DMEM culture fluid, pancreatic enzyme digestion. Cells were laid in a 12 mm round cover glass, 24-well plate culture.
[0027] The film wrench technology records the cell membrane current: the diaphragm pliers amplifier adopts HEKA-EPC10. Both mymium B (final concentration 0.1 to 0.2 mg / ml) is used in the electrode. After the microelectrode polishes, the inner liquid in which the electrode is applied, and the resistance value is controlled at 2 to 4 MΩ. Electrode induced by (mm): KCl 160, HEPES 5, MGCL 2 3, CACL 2 1, EGTA 3, use KOH to adjust pH to 7.4; extracellular fluid components (mm): NaCl 160, KCl 2.5, HEPES 10, Glucose8, MGCL 2 1, CACL 2 5. After the microelectrode is sealed with the cell membrane, ...
Embodiment 2
[0031] Pharmacodynamics experiment of houtu saffin sodium
[0032] Pentylphenazole PTZ induces seizures:
[0033]Experimental medication: 2% Tween 20 suspension of Sodium Houttuyfonate, the control group used 2% Tween 20 mixer of Ruckill Plus. Experimental method: Each group of male mice induced epilepsy by intraperitoneal injection of glutarazole, observed that epilepsy latency during 90 minutes, seizures, seizures, and epilepsy level. The peritoneal injection of napper sodium is 12.5 mg / kg, 25 mg / kg, 50 mg / kg, and the control group is 15 mg / kg. The experimental results are shown in Table 1, and sodium fish, sodium can significantly reduce the duration of epilepsy, and seizures and seizures. In summary, the sodium of hookuemen is significantly anti-epilepsy.
[0034] Table 1: The sodium of Houttuynia is significantly reduced the continuous episode of epileptic seizures, the number of epilepsy, the level of epilepsy, and mortality.
[0035]
[0036] Note: * The significa...
Embodiment 3
[0039] The tablet is prepared according to the method known in the art, and each piece contains the following ingredients:
[0040] 50 mg of sodium fish, 70 mg of lactose, 3 mg of stearate, and 130 mg of polyvinylpyrrolidone.
[0041] Capsules are prepared according to methods known in the art, and each capsule contains the following ingredients:
[0042] Hydae is 50 mg, lactose 70 mg, corn starch 25 mg, stearate 1 mg, polyvinylpyrrolidone 130 mg.
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