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Recombinant exosome nano vaccine

A nano-vaccine and exosome technology, applied in the field of nano-vaccine of recombinant exosomes, can solve the problems of not making good use of nano-level technology.

Inactive Publication Date: 2021-06-11
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The common methods and public technologies under the existing technology do not make good use of nano-level technology and use it in vaccine technology. With the global health situation, the application of nano-level exosomes is essential

Method used

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  • Recombinant exosome nano vaccine
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Embodiment Construction

[0012] Next, the technical solutions in the embodiments of the present invention will be apparent from the embodiment of the present invention, and it is clearly described, and it is understood that the described embodiments are merely embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, there are all other embodiments obtained without making creative labor without making creative labor premises.

[0013] See Figure 1 to 2 The present invention provides a technical solution: a nano-vaccine of recombinant outer body, including S proteins and VSV-G vectors, and the VSV-G vector is expressed after AAV-293 cell eukaryotes, S protein will be inserted by vsvg connection. Go to the surface of the outer membrane.

[0014] Subrextraroduonts (1-1195) polynucleotides have been optimized, such as SEQ ID NO: 1. SEQ IDNO: 1 Sequence See Annex.

[0015] Optimized to optimize the original S protein gene sequence, most of the rare cod...

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Abstract

The invention relates to the technical field of exosomes, in particular to a recombinant exosome nano vaccine which comprises S protein and a VSV-G vector. After the VSV-G vector is subjected to eukaryotic expression in AAV-293 cells, the S protein can be inserted into the surface of an exosome membrane in a VSVG connection mode. The preparation method has the beneficial effects that the advantages of a recombinant protein vaccine and a nano vaccine are integrated, the S protein and the VSVG are fused, expressed and inserted into the surface of an exosome membrane, the S protein is carried on the nano-scale exosome secreted by cells, and an organism can be stimulated to generate an antibody by injecting the S protein into the organism. The nano vaccine has the advantages of safety, high efficiency and large-scale production.

Description

Technical field [0001] The present invention relates to the field of outer body, and is a nanotonic nanopathy of recombinant outer body. Background technique [0002] Natural nanocapsulas circulating in the human body have an intrinsic ability of various proteins into receptor cells, an efficient delivery carrier. The project is used to perform antigen protein display and delivery in the project, which is expected to use a live carrier as a therapeutic agent to the cells of mammalian cells. [0003] The common means of the prior art and disclosure techniques are not well utilized to use nano-level technologies, and they are used in vaccine techniques, and with global health conditions, nano-level exose applications are essential. Inventive content [0004] It is an object of the present invention to provide a nano-vaccine of recombinant outer body to solve the objects mentioned in the background technique to achieve technical applications of nanotechnology in vaccine preparation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K47/46A61P31/14B82Y5/00C12N15/86
CPCA61K39/12A61K47/46A61K2039/54A61P31/14B82Y5/00C12N15/86C12N2760/20243C12N2770/20034C12N2800/107C12N2800/22
Inventor 梁宇杰段莉徐丽梅徐晓
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN