Microflow pipeline for gathering non-spherical micro swimming bodies and filtering method

A swimming body, non-spherical technology, applied in the field of microfluidics, can solve the problems of difficult construction and blockage of microfluidic pipelines, achieve the effect of simple structure and reduce the probability of pipeline blocking

CN112973815AActive Publication Date: 2021-06-18NORTHWEST UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2021-06-18

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Abstract

The invention discloses a microflow pipeline for gathering non-spherical micro swimming bodies and a filtering method, and relates to the field of microflow, in particular to the microflow pipeline for gathering the non-spherical micro swimming bodies and the filtering method, the microflow pipeline comprises a microflow pipeline body, and an expansion pipe used for forming an expansion flow field is arranged on the microflow pipeline body; the expansion tube comprises a head end expansion tube connecting section and a tail end connecting section; the two ends of the expansion pipe are respectively communicated with the micro-flow pipeline bodies positioned on the two sides of the expansion pipe through a head end expansion pipe connecting section and a tail end connecting section; and the cross section of the head end expansion pipe connecting section is continuously increased in the fluid flowing direction. The microflow pipeline is simple in structure, by means of the expansion pipe which is connected to the micro-flow pipeline body and used for forming an expansion flow field, the micro-swimming bodies in fluid are enriched in the expansion pipe, and therefore collection or filtering of the micro-swimming bodies is facilitated.
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Description

technical field

[0001] The invention relates to the field of microfluidics, in particular to a microfluidic channel for gathering non-spherical microswimming bodies and a filtering method. Background technique

[0002] Microswimming bodies refer to individuals with a size ranging from submillimeters to microns that have their own driving force and can swim in a flow field, including bacteria, sperm, and micro-robots.

[0003] Due to the application of microswimmers, the existing fluid environments are mostly dynamic and complex, and how to separate such individuals from fluids is challenging. The general membrane filtration method can only separate individuals with different sizes, and it is difficult to construct in a microfluidic channel with a small size, and it is easy to cause blockage. Developing a method of collecting and separating microswimming bodies based on individual swimming ability by using a flow field is of great significance and commercial value in the fie...

Examples

example 1

[0053]By photolithography on silicon wafers such as Figure 5 A microfluidic channel with aggregated non-spherical micro-swimming bodies, d=50 microns, α=45 degrees, is made into a PDMS channel with a thickness of 50 microns through reverse molding.

[0054] The Escherichia coli RP437 solution with an OD value of 0.1 was passed into the microfluidic channel, and the movement speed of RP437 was 20 μm / s.

[0055] Scanning in the flow range from 0.16 nL / s to 2.5 nL / s, it was found that the number density of aggregated regions was the highest at 0.5 nL / s.

[0056] Fix the flow rate to 0.5 nL / s, and the micrograph after 1 minute is as follows Figure 6 As shown, at this time, the inner area of ​​the connecting section 3 of the expansion tube at the head end is the area of ​​the expansion flow field, as shown in Figure 6 As shown in the dashed box, the number density in this area is 3.7 times the average number density in the pipeline under the condition of no flow velocity.

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