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Method for producing rare sugar-containing composition and rare sugar-containing composition

A rare sugar and composition technology, applied in medical preparations containing active ingredients, biochemical equipment and methods, medical preparations with non-active ingredients, etc., can solve problems such as the difficulty in mass production of D-psicose

Pending Publication Date: 2021-06-22
KAGAWA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of carrying out the manufacture of refined D-fructose and then the manufacture of D-psicose, since it is carried out in separate processes, it is subject to constraints such as raw materials, transportation, reaction costs, and equipment operating costs. In reality, D- Industrial mass production of allulose is very difficult

Method used

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  • Method for producing rare sugar-containing composition and rare sugar-containing composition
  • Method for producing rare sugar-containing composition and rare sugar-containing composition
  • Method for producing rare sugar-containing composition and rare sugar-containing composition

Examples

Experimental program
Comparison scheme
Effect test

manufacture example

[0170] The above-mentioned food and drink can also be used as functional food, nutritional supplement food or health food. Its form is not particularly limited. For example, as an example of food production, proteins such as milk protein, soybean protein, and ovalbumin with high nutritional value and balanced amino acid balance, their decomposition products, egg white oligopeptides, soybean protein, etc., can be used in accordance with conventional methods. Hydrolyzate and other mixtures of amino acid monomers, etc. Moreover, it can also utilize in the form of a soft capsule, a tablet, etc.

[0171] Examples of nutritional supplements or functional foods include liquid foods, semi-digested nutritional foods, ingredient nutritional foods, health drinks, and capsules containing carbohydrates, fats, trace elements, vitamins, emulsifiers, fragrances, etc. , enteral nutrition and other processing forms. In the above-mentioned various foods, such as sports drinks, nutritional drin...

Embodiment 1

[0187] Generally, when using isomerase to produce rare sugars, there are:

[0188] (1) Cultivation of microorganisms and mass production of enzymes,

[0189] (2) conversion to rare sugar by enzymatic reaction,

[0190] (3) Separation of the rare sugar of the product and the raw material (fructose, etc.) during the equilibrium reaction,

[0191] (4) The 4th process of concentrating a rare sugar, if necessary, the 5th process of (5) crystallization of a rare sugar is added. These processes are carried out efficiently to produce rare sugar in large quantities.

[0192] An example of a preliminary experiment is shown in this Example.

[0193] The production method of the preliminary experiment is characterized in that the separation of rare sugars and raw materials (fructose, etc.) in the above (3) is not performed.

[0194] That is, if figure 1 As shown, the normal production of D-psicone from D-fructose in Reaction 1 where ketose 3-epimerase acts on D-fructose as a raw mate...

Embodiment 2

[0214] It was shown that by changing the activity (enzyme amount) of D-xylose isomerase in the second reaction, the ratio of D-glucose:D-fructose:D-psicose can be changed according to the target.

[0215] Another major feature of the present invention is that the composition of the product is not fixed, and a composition suitable for the purpose can be obtained. It was confirmed that products of various compositions can be obtained by changing the reaction conditions. By varying the enzymatic activity of the two enzymes (amount of immobilized enzyme) or the speed of passage through the column of immobilized enzymes, products of varying composition can be produced. This example shows the possibility by varying the amount of enzyme.

[0216] Hereinafter, the characteristics of the present invention will be investigated by changing the activity (enzyme amount) of D-xylose isomerase in the second reaction and measuring the composition of the product.

[0217] First, ketose 3-epi...

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Abstract

To provide a rare sugar-containing composition that maintains the excellent functionality of D-psicose while taking into consideration the need to be improved as a sweetener. A method for producing a rare sugar-containing composition using D-fructose as a feedstock, said method being characterized in that a target product comprising a novel composition of D-glucose, D-fructose and D-psicose is obtained by producing a mixture of D-fructose in ketose 3-epimerase and D-psicose in a first stage, and converting D-fructose using D-xylose isomerase, which does not act on D-psicose, into D-glucose in a second stage. The product of the first stage is a mixture of D-fructose and a rare sugar D-psicose. This mixture is a sweetener having the same taste as sugar.

Description

technical field [0001] The present invention relates to a method of using D-fructose as a raw material, making the enzyme reaction into two steps, and combining two enzyme reactions in series, so that it can not be lost due to separation operations in the manufacturing process, and in principle, 100% of all raw materials are used as A method for producing a sugar-containing composition obtained as a product, a rare sugar-containing composition having the same taste as granulated sugar as a product, and uses thereof. [0002] Here, the enzymes ketose 3-epimerase and xylose isomerase used in the present invention are defined. [0003] (1) Isomerase [0004] Isomerases catalyze "reactions in which the chemical formula does not change". Isomerase acting on hexose catalyzes "Chemical Formula C 6 h 12 o 6 reactions in which no change occurs". For example, aldose isomerase catalyzes the "movement of intramolecular hydrogen," advancing the C 1 with C 2 redox reactions between t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/02A23L27/00C12N9/90C12N9/92
CPCC12N9/90C12N9/92C12P19/02A23L29/30A23L33/125C12Y501/03C12Y503/01005A61K31/7004A61P3/04A61P3/10A61K8/60A61Q19/00A61Q11/00A23L33/26A23L27/30A23V2002/00A23V2200/322A23V2250/606A23V2250/61A23L27/33A61K47/26C12P19/24
Inventor 秋光和也何森健高冈晴造吉原明秀加藤志郎望月进
Owner KAGAWA UNIVERSITY
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